A novel sandwich enzyme-linked immunosorbent assay for feline insulin.
A novel sandwich enzyme-linked immunosorbent assay for feline insulin.
复制标题
一种新型夹心酶联免疫吸附测定猫胰岛素。
DOI：
10.1292/jvms.71.1005
复制
发表时间：
2009
影响因子：
1.2
通讯作者：
K. Ono
中科院分区：
文献类型：
--
作者：
Mariko Nakaya;Yuko Kito;N. Matsuki;H. Shibata;Yuki Touhata;S. Tamahara;K. Ono
建立了测定家猫血清胰岛素浓度的双抗体夹心酶联免疫吸附试验（ELISA）。通过使用固相小鼠抗牛胰岛素单克隆抗体和过氧化物酶结合的豚鼠抗大鼠胰岛素多克隆抗体，可以测量猫血清胰岛素浓度在0.1至3.6 ng/ml的范围内。批内CV <6%，批间CV <10%。本发明的胰岛素测定将有力地帮助猫糖尿病的研究。
A novel sandwich enzyme-linked immunosorbent assay (ELISA) was established to determine the serum insulin concentrations in domestic cats. By using a solid-phase mouse anti-bovine insulin monoclonal antibody and a peroxidase-conjugated guinea pig anti-rat insulin polyclonal antibody, feline serum insulin concentrations in the range of 0.1 to 3.6 ng/ml could be measured. The intraassay CV and interassay CV were less than 6% and less than 10%, respectively. The present insulin assay will strongly help studies on feline diabetes mellitus.