Facilitated DNA transfer to rat submandibular gland in vivo and GRP-Ca gene regulation.
Facilitated DNA transfer to rat submandibular gland in vivo and GRP-Ca gene regulation.
复制标题
促进 DNA 体内转移至大鼠颌下腺和 GRP-Ca 基因调控。
DOI：
10.1152/ajpgi.1995.268.6.g1074
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Baum,BJ
中科院分区：
文献类型：
--
作者：
O'Connell,BC;TenHagen,KG;Lazowski,KW;Tabak,LA;Baum,BJ
The internalization of DNA can be facilitated by adenovirus infection. Using the replication-deficient adenovirus, Ad-dl312, and a plasmid-based firefly luciferase gene as a reporter, we have optimized the uptake and expression of DNA in rat submandibular glands in vivo. Luciferase expression is transient and peaked at approximately 18 h after infection. Luciferase activity increased with plasmid concentration and was greatest at 10(9) to 10(10) plaque-forming units of Ad-dl312 per gland. We next examined the expression in vivo of plasmids containing deletions of the glutamine/glutamic acid-rich protein (GRP-Ca isoform) gene upstream region linked to a chloramphenicol acetyltransferase (CAT) reporter. Constructs with 9.4, 6.3, and 2.7 kb and 17 base pairs of upstream sequence gave relative CAT activities of 100, 30, 7.6, and 38.5, respectively. With the 9.4-kb GRP-Ca construct, CAT was preferentially expressed in acinar cells, which is characteristic of GRP. This gene transfer approach should prove useful in the further study of gene expression in salivary glands and other organs.
