A multilocus sequence typing scheme for Streptococcus pneumoniae:: identification of clones associated with serious invasive disease
A multilocus sequence typing scheme for Streptococcus pneumoniae:: identification of clones associated with serious invasive disease
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DOI：
10.1099/00221287-144-11-3049
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发表时间：
1998-11-01
期刊：
影响因子：
--
通讯作者：
Spratt, BG
中科院分区：
文献类型：
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作者：
Enright, MC;Spratt, BG
肺炎链球菌的种群生物学知之甚少。大多数重要的问题可以通过对来自携带和来自肺炎球菌疾病的不同表现的大量、良好采样的人群进行分子表征来解决。因此，作者开发了一个肺炎球菌多位点序列分型方案和数据库，通过测序类似的450 bp片段的7个管家基因座从295株。在7个位点的等位基因的组合提供了一个等位基因谱，或序列类型（ST），和菌株之间的相关性，通过构建一个树状图从ST之间的成对差异矩阵。分型方案使用已知遗传相关性的肺炎球菌进行验证，并且可以解析> 60亿ST。在来自8个国家的最近侵袭性肺炎球菌疾病病例的274个分离株中，解析了143个ST，但是12个ST包含至少5个分离株（范围5-21个分离株）。在不同国家反复从侵袭性疾病中回收无法区分的分离株，这意味着这些ST定义的菌株引起侵袭性疾病的能力增加。ST和血清型之间的关系表明，从长远来看，荚膜基因已水平分布在肺炎球菌种群，但在短期内，克隆的扩张发生只有偶尔的血清型的变化。多位点序列分型方案提供了一种强有力的新方法来表征肺炎球菌，因为它提供了分子分型数据，这些数据在实验室之间是电子便携式的，并且可以用于探测这些生物体的群体和进化生物学方面。通过MLST对肺炎球菌进行分子表征的网站是可用的（http：mlst.zoo.ox.ac.uk）。
The population biology of Streptococcus pneumoniae is poorly understood. Most of the important issues could be addressed by the molecular characterization of large, well sampled populations from carriage and from the different manifestations of pneumococcal disease. The authors have therefore developed a pneumococcal multilocus sequence typing scheme and database by sequencing similar to 450 bp fragments of seven housekeeping loci from 295 isolates. The combination of alleles at the seven loci provided an allelic profile, or sequence type (ST), and the relatedness between isolates was obtained by constructing a dendrogram from the matrix of pairwise differences between STs. the typing scheme was validated using pneumococci of known genetic relatedness and could resolve >6 billion STs, Among 274 isolates from recent cases of invasive pneumococcal disease in eight countries, 143 STs were resolved, but 12 STs contained at least five isolates (range 5-21 isolates). The repeated recovery of indistinguishable isolates from invasive disease in different countries implies that these STs define strains with an increased capacity to cause invasive disease. The relationship between STs and serotypes suggested that, in the longer term, capsular genes have been distributed horizontally within the pneumococcal population, but in the short term, expansion of clones occurs with only occasional changes of serotype. The multilocus sequence typing scheme provides a powerful new approach to the characterization of pneumococci, since it provides molecular typing data that are electronically portable between laboratories, and which can be used to probe aspects of the population and evolutionary biology of these organisms. A Web site for the molecular characterization of pneumococci by MLST is available (http://mlst.zoo.ox.ac.uk).