Effects of ammonia exposure on apoptosis, oxidative stress and immune response in pufferfish (Takifugu obscurus)
Effects of ammonia exposure on apoptosis, oxidative stress and immune response in pufferfish (Takifugu obscurus)
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DOI：
10.1016/j.aquatox.2015.04.004
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发表时间：
2015-07-01
影响因子：
4.5
通讯作者：
Wang, An-Li
中科院分区：
文献类型：
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作者：
Cheng, Chang-Hong;Yang, Fang-Fang;Wang, An-Li
氨是淡水水生系统中影响生物生存和生长的主要环境污染物之一。本文研究了氨暴露对暗纹东方鲀细胞凋亡、氧化应激和免疫应答的影响。将鱼暴露于各种浓度的氨（0、1.43、3.57、7.14 mM）72 h。结果表明，氨暴露可诱导细胞内活性氧自由基（reactive oxygen species，ROS）的产生，破坏细胞内Ca 2+（cf-Ca 2+）稳态，导致DNA损伤和细胞凋亡。为了检测凋亡途径，检测了一些关键凋亡相关基因包括P53、Bax Bcl 2、Caspase 9、Caspase 8和Caspase 3在肝脏中的表达模式。结果表明，氨胁迫可改变这些基因的转录，并导致细胞凋亡增加，提示氨胁迫诱导的细胞凋亡可能与P53-Bcl 2途径和caspase依赖的凋亡途径有关。此外，氨胁迫还可诱导炎性细胞因子（BAFF、TNF-α、IL-6和IL-12）转录水平上调，表明先天免疫系统在氨诱导的鱼类毒性中起重要作用。此外，氨胁迫诱导了肝脏中抗氧化酶（Mn-SOD、CAT、GPx和GR）和热休克蛋白（HSP 90和HSP 70）的基因表达，表明抗氧化系统和热休克蛋白试图保护细胞免受氨胁迫诱导的氧化应激和凋亡。本研究结果将有助于了解氨对鱼类的水毒性机制。(C)2015 Elsevier B. V.版权所有。
Ammonia is one of major environmental pollutants in the freshwater aquatic system that affects the survival and growth of organisms. In the present study, we investigated the effects of ammonia exposure on apoptosis, oxidative stress and immune response in pufferfish (Takifugu obscurus). Fish were exposed to various concentrations of ammonia (0, 1.43, 3.57, 7.14 mM) for 72 h. The date showed that ammonia exposure could induce intracellular reactive oxygen species (ROS), interrupt intracellular Ca2+ (cf-Ca2+) homeostasis, and subsequently lead to DNA damage and cell apoptosis. To test the apoptotic pathway, the expression patterns of some key apoptotic related genes including P53, Bax Bcl2, Caspase 9, Caspase 8 and Caspase 3 in the liver were examined. The results showed that ammonia stress could change these genes transcription, associated with increasing of cell apoptosis, suggesting that the P53-Bax-Bcl2 pathway and caspase-dependent apoptotic pathway could be involved in cell apoptosis induced by ammonia stress. In addition, ammonia stress could induced up-regulation of inflammatory cytokines (BAFF, TNF-alpha, IL-6 and IL-12) transcription, indicating that innate immune system play important roles in ammonia-induced toxicity in fish. Furthermore, the gene expressions of antioxidant enzymes (Mn-SOD, CAT, GPx, and GR) and heat shock proteins (HSP90 and HSP70) in the liver were induced by ammonia stress, suggesting that antioxidant system and heat shock proteins tried to protect cells from oxidative stress and apoptosis induced by ammonia stress. Our results will be helpful to understand the mechanism of aquatic toxicology induced by ammonia in fish. (C) 2015 Elsevier B.V. All rights reserved.