CONDUCTION STUDIES IN PERIPHERAL CAT NERVE USING IMPLANTED ELECTRODES .2. THE EFFECTS OF PROLONGED CONSTRICTION ON REGENERATION OF CRUSHED NERVE-FIBERS
CONDUCTION STUDIES IN PERIPHERAL CAT NERVE USING IMPLANTED ELECTRODES .2. THE EFFECTS OF PROLONGED CONSTRICTION ON REGENERATION OF CRUSHED NERVE-FIBERS
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DOI：
10.1002/mus.880110906
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发表时间：
1988-09-01
期刊：
影响因子：
3.4
通讯作者：
PEZESHKPOUR, GH
中科院分区：
文献类型：
--
作者：
KRARUP, C;LOEB, GE;PEZESHKPOUR, GH
长期植入的电极阵列用于检查近端小腿胫神经挤压后猫周围神经纤维伸长和成熟的时间过程。将单独挤压后的再生与神经紧缩近端挤压 5 毫米进行比较。通过沿着胫骨和足底神经上的电极阵列的兴奋性进展来监测再生。灵敏度足以记录单个神经纤维的平均活动，从而可以检测最早的再生。最快再生纤维的直径是根据挤压部位附近的传导速度来估计的。无论是单独挤压后还是挤压收缩后，小有髓纤维都在大纤维前面再生。单独挤压后的伸长率为 3.2 毫米/天，而挤压 + 收缩远端的伸长率较慢 (P < 0.02) (2.2 毫米/天)。在这两个损伤中，再生开始的推断延迟为 8 天。在挤压后长达300天的观察中，成熟因收缩而延迟或受损，并且复合神经动作电位具有较小的幅度和分散的形状。与单独挤压相比，挤压+收缩后的神经横截面显示大纤维数量减少，小纤维数量增加，这可能是由于再生纤维的持续分支所致。单独挤压和挤压+收缩后，再生纤维具有相似的g比，表明髓鞘化在直径减小的纤维中完全发育。
Arrays of chronically implanted electrodes were used to examine the time course of elongation and maturation of peripheral nerve fibers in the cat after crush of the tibial nerve in the proximal calf. Regeneration after crush alone was compared with crush 5 mm proximal to a tight constriction of the nerve. Regeneration was monitored by the progression of excitability along the electrode arrays on the tibial and plantar nerves. The sensitivity was sufficient to record the averaged activity in single nerve fibers allowing detection of the earliest regeneration. The diameters of the fastest regeneration fibers were estimated from the conduction velocity proximal to the site of crush. Both after crush alone, and after crush constriction, small myelinated fibers regenerated in front of large fibers. The rate of elongation after crush alone was 3.2 mm/day, whereas it was slower (P < 0.02) distal to crush + constriction (2.2 mm/day). In both lesions, the extrapolated delay to onset of regeneration was 8 days. In observations up to 300 days after crush, maturation was delayed or impaired by the constriction, and the compound nerve action potential had a smaller amplitude and a dispersed shape. Transverse sections of nerves after crush + constriction showed a diminished number of large and an increased number of small fibers compared with crush alone, possibly due to persistent branching of regenerated fibers. After both crush alone and crush + constriction, regenerated fibers had similar g ratios, suggesting that myelination developed fully in fibers of diminished diameters.