Potential of Dental Mesenchymal Cells in Developing Teeth
Potential of Dental Mesenchymal Cells in Developing Teeth
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DOI：
10.1634/stemcells.2006-0360
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发表时间：
2007-01
期刊：
影响因子：
5.2
通讯作者：
H. Yamazaki;M. Tsuneto;M. Yoshino;K. Yamamura;S. Hayashi
中科院分区：
文献类型：
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作者：
H. Yamazaki;M. Tsuneto;M. Yoshino;K. Yamamura;S. Hayashi
牙齿由牙本质和釉质组成，通过上皮-间充质相互作用发育。神经嵴（NC）细胞在发育中的牙齿中形成牙间充质，并分化为分泌牙本质的成牙本质细胞。已知NC细胞在颅面区域中分化成软骨细胞和成骨细胞。然而，尚不清楚发育中的牙齿中的牙齿间充质细胞是否具有分化成牙本质细胞谱系以外的谱系的潜力。在这项研究中，我们从E13.5牙胚细胞制备间充质细胞，并评估其在培养中的分化潜力。它们分化成牙本质细胞、软骨细胞样细胞和成骨细胞样细胞。通过使用P0-Cre/Rosa 26 R小鼠将NC-衍生细胞追踪为LacZ+细胞来确认其来源。使用流式细胞术-荧光二-β-d-半乳糖苷酶系统，这使得可以检测LacZ+细胞作为活细胞，表征了牙齿间充质细胞的细胞表面分子。大量LacZ+ NC衍生细胞表达血小板衍生生长因子受体α和整合素。总之，这些结果表明，具有分化为软骨细胞样和成骨细胞样细胞的潜力的NC衍生细胞存在于发育中的牙齿中，这些细胞可能有助于牙齿器官的形成。
The tooth, composed of dentin and enamel, develops through epithelium‐mesenchyme interactions. Neural crest (NC) cells contribute to the dental mesenchyme in the developing tooth and differentiate into dentin‐secreting odontoblasts. NC cells are known to differentiate into chondrocytes and osteoblasts in the craniofacial region. However, it is not clear whether the dental mesenchymal cells in the developing tooth possess the potential to differentiate into a lineage(s) other than the odontoblast lineage. In this study, we prepared mesenchymal cells from E13.5 tooth germ cells and assessed their potential for differentiation in culture. They differentiated into odontoblasts, chondrocyte‐like cells, and osteoblast‐like cells. Their derivation was confirmed by tracing NC‐derived cells as LacZ+ cells using P0‐Cre/Rosa26R mice. Using the flow cytometry‐fluorescent di‐β‐d‐galactosidase system, which makes it possible to detect LacZ+ cells as living cells, cell surface molecules of dental mesenchymal cells were characterized. Large number of LacZ+ NC‐derived cells expressed platelet‐derived growth factor receptor α and integrins. Taken together, these results suggest that NC‐derived cells with the potential to differentiate into chondrocyte‐like and osteoblast‐like cells are present in the developing tooth, and these cells may contribute to tooth organogenesis.