Antibacterial and antioxidant effects of Rosmarinus officinalis L. extract and its fractions
Antibacterial and antioxidant effects of Rosmarinus officinalis L. extract and its fractions
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DOI：
10.1016/j.jtcme.2017.10.006
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发表时间：
2019-10-01
影响因子：
4.5
通讯作者：
Fachinetto, Roselei
中科院分区：
文献类型：
--
作者：
Amaral, Guilherme Pires;Mizdal, Caren Rigon;Fachinetto, Roselei
超过与病原菌相关的生理水平的活性物质的产生可能代表许多疾病的高风险。迷迭香Rosmarinus officinalis L.由于其药理学特性而在世界各地使用。因此，在这项研究中，我们的目标是测试第一次，如果R。officinalis L.提取物（eeRo）及其组分（DCM、EA、ButOH）在体外和离体实验中，对大鼠脑、胃和肝的抗氧化作用均优于或接近标准品和同类组分。此外，我们打算阐明它们对致病菌的可能影响。eeRo从经受醇提取和分级的干燥叶获得。通过HPLC对eeRo和级分的成分进行定量。研究了R.采用DPPH中心点自由基清除试验、总抗氧化试验、二氯荧光素试验、脂质过氧化试验和硝普钠诱导的脂质过氧化试验，分析了当归的抗氧化活性。最低抑菌浓度（Minimum inhibitory concentrations of R. officinalis L.用危险细菌的标准菌株进行测试。eeRo、DCM、EA具有显著的总抗氧化活性和DPPH中心点自由基清除活性。DCM和eeRo对肝、胃和脑中反应性物质的基础水平有显著影响。eeRo和DCM对肝和脑的脂质过氧化有保护作用。eeRo、DCM、EA和ButOH对革兰氏阳性菌和革兰氏阴性菌均有抑制作用。总的来说，DCM和eeRo的抗氧化和抑菌效果最好。(C)2017台湾大学食品与生物分子研究中心由爱思唯尔台湾有限责任公司制作和主办。
The production of reactive species over physiological levels associated to pathogenic bacteria could represent a high risk for many diseases. The Rosmarinus officinalis L. is used around the world due its pharmacological proprieties. So, in this study our aim is to test for the first time if R. officinalis L. extract (eeRo) and its fractions (DCM, EA, ButOH) could have better or similar antioxidant action to standars and among themselves in vitro or ex vivo, in brain, stomach and liver of rats. Moreover, we intend to clarify their possible effects on pathogenic bacteria. The eeRo was obtained from the dried leaves subjected to an alcoholic extraction and fractioned. The quantification of the constituents of eeRo and fractions were done by HPLC. The antioxidant proprieties of R. officinalis was analyzed by DPPH center dot(-) radical scavenging, total antioxidant, dichlorofluorescein, lipid peroxidation and sodium nitroprusside -induced lipid peroxidation assays. The Minimum inhibitory concentrations of R. officinalis L. were tested with standard strains of danger bacteria. The eeRo, DCM, EA had significant total antioxidant and DPPH center dot(-) radical scavenging activities. The DCM and eeRo got significant effects against basal levels of reactive species in liver, stomach and brain. The eeRo and DCM protected the liver and brain against lipid peroxidation. The eeRo, DCM, EA and ButOH had inhibitory effect in the Gram-positive and Gram-negative bacteria. In general way, the DCM and eeRo had the best antioxidant and antibacterial effects among all tested fractions. (C) 2017 Center for Food and Biomolecules, National Taiwan University. Production and hosting by Elsevier Taiwan LLC.