Studies on the conversion of prothrombin to thrombin: with notes on the cation requirement for this reaction.
Studies on the conversion of prothrombin to thrombin: with notes on the cation requirement for this reaction.
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关于凝血酶原转化为凝血酶的研究：附有该反应所需阳离子的注释。
DOI：
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发表时间：
1967
期刊：
影响因子：
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通讯作者：
O. Ratnoff
中科院分区：
文献类型：
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作者：
C. Prentice;R. Breckenridge;O. Ratnoff
摘要：本研究探讨了导致凝血酶前体凝血酶原形成凝血酶的反应。数据表明，由凝血酶原和“凝血酶原转化原理”的混合物产生的凝血酶的数量与最初存在的凝血酶原的数量直接相关。“凝血酶原转化原理”是在磷脂和钙离子存在的情况下，由活化的斯图尔特因子和加速素相互作用产生的。然而，凝血酶的生成速率是“凝血酶原转化原理”浓度的函数，并且在0°c的温度下大大延迟。这些数据提供了实验证据，证明凝血酶原的激活是酶促的，这是许多研究者长期持有的观点。在本研究中使用的条件下，凝血酶必须存在钙或锶离子才能从凝血酶原进化而来。
Abstract The present study examines the reaction leading to the formation of thrombin from its precursor, prothrombin. The data demonstrate that the amount of thrombin generated from a mixture of prothrombin and the "prothrombin-converting principle," derived from the interaction of activated Stuart factor and proaccelerin in the presence of phospholipid and calcium ions, is directly related to the amount of prothrombin initially present. The rate of generation of thrombin, however, is a function of the concentration of the "prothrombin-converting principle," and is greatly retarded at a temperature of 0 ° C. These data provide experimental evidence that the activation of prothrombin is enzymatic, a view long held by many investigators. The cations calcium or strontium must be present for thrombin to evolve from prothrombin under the conditions used in this study.