OBLIGATORY AND PERIOD SPECIFIC REQUIREMENT OF IRON FOR MICROSPORE EMBRYOGENESIS IN DATURA-METEL ANTHER CULTURES
OBLIGATORY AND PERIOD SPECIFIC REQUIREMENT OF IRON FOR MICROSPORE EMBRYOGENESIS IN DATURA-METEL ANTHER CULTURES
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DOI：
10.1007/bf02488823
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发表时间：
1986-06-01
期刊：
影响因子：
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通讯作者：
GUPTA, SC
中科院分区：
文献类型：
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作者：
BABBAR, SB;GUPTA, SC
在曼陀罗花药培养中，铁在小孢子植株形态发生中的作用的实验表明，即使在缺铁的培养基上，雄核发育也开始了，但如果花药不转移到含铁的培养基上，诱导的单位不能生长超过多细胞阶段。另一个重要的结论来自于一个实验，该实验涉及从缺乏铁源（硫酸亚铁，FeSO 4·7 H2O，10-4 M）和螯合物（乙二胺四乙酸的二钠盐）的营养培养基中每周转移花药。C10 H14 N2 Na 2 O 8·2 H2O，10-4 M）与含有这两者的培养基或反之亦然的差异在于，如果在初始培养期间存在铁，则实际上不利地影响小植株产量。因此，培养在缺铁培养基上的花药，为最初的一个或两个星期，然后转移到含铁培养基，产生了更多的小植株比那些从花药培养连续完全的基础培养基具有铁和螯合物。
The experiments to assess the role of iron in morphogenesis of microspore-derived plantlets in anther cultures of Datura metel revealed that even on iron-deficient medium androgenesis is initiated but the induced units fail to grow beyond multicellular stage, if anthers are not transferred to iron-incorporated medium. Another important conclusion drawn from an experiment involving weekly transfer of anthers from nutrient medium lacking both iron source (ferrous sulfate, FeSO4.cntdot.7H2O, 10-4 M) and chelate (disodium salt of ethylene diamine tetraacetic acid. C10H14N2Na2O8.cntdot.2H2O, 10-4 M) to that containing both of these or vice-versa, is that iron if present during the initial culture period, in fact affects the plantlet yield adversely. Thus, anthers cultured on iron-deficient medium, for initial one or two weeks and then transferred to iron-containing medium, yielded higher number of plantlets than those developed from anthers cultured continuously on complete basal medium having both iron and chelate.