Two featured series of rRNA-derived RNA fragments (rRFs) constitute a novel class of small RNAs.
Two featured series of rRNA-derived RNA fragments (rRFs) constitute a novel class of small RNAs.
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rRNA 衍生的 RNA 片段 (rRF) 的两个特征系列构成了一类新型小 RNA
DOI：
10.1371/journal.pone.0176458
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Gao S
中科院分区：
文献类型：
--
作者：
Chen Z;Sun Y;Yang X;Wu Z;Guo K;Niu X;Wang Q;Ruan J;Bu W;Gao S
在这项研究中，我们使用illumima平台报道了两个来自雄性羊僵菌（Amblyomma testudinarium）小RNA测序（sRNA-seq）数据的rrna衍生RNA片段（rRFs）系列。两个系列的rrf （rRF5和rRF3）精确地排列在5.8S和28S rRNA基因的5‘和3’端。rRF5和rRF3系列的表达量明显高于rRNA基因中位于体内的rrf。这些序列包含完全对齐的reads，其长度以1bp的差异逐渐变化。具有相同表达模式的rRF5和rRF3系列从蜱到人都普遍存在。细胞实验表明，RNAi敲低一个20-nt rRF3可诱导细胞凋亡，抑制细胞增殖。此外，RNAi敲低导致H1299细胞在细胞周期的G2期显著减少。这些结果表明，rRF5和rRF3系列不是rRNA降解过程中的随机中间体或产物，而是可能构成一类新的小rna，值得进一步研究。
In this study, we reported two featured series of rRNA-derived RNA fragments (rRFs) from the small RNA sequencing (sRNA-seq) data of Amblyomma testudinarium using the Illunima platform. Two series of rRFs (rRF5 and rRF3) were precisely aligned to the 5' and 3' ends of the 5.8S and 28S rRNA gene. The rRF5 and rRF3 series were significantly more highly expressed than the rRFs located in the body of the rRNA genes. These series contained perfectly aligned reads, the lengths of which varied progressively with 1-bp differences. The rRF5 and rRF3 series in the same expression pattern exist ubiquitously from ticks to human. The cellular experiments showed the RNAi knockdown of one 20-nt rRF3 induced the cell apoptosis and inhibited the cell proliferation. In addition, the RNAi knockdown resulted in a significant decrease of H1299 cells in the G2 phase of the cell cycle. These results indicated the rRF5 and rRF3 series were not random intermediates or products during rRNA degradation, but could constitute a new class of small RNAs that deserves further investigation.