Transcriptome sequencing and metabolite analysis reveals the role of delphinidin metabolism in flower colour in grape hyacinth.
Transcriptome sequencing and metabolite analysis reveals the role of delphinidin metabolism in flower colour in grape hyacinth.
复制标题
转录组测序和代谢物分析揭示飞燕草素代谢对葡萄风信子花色的作用
DOI：
10.1093/jxb/eru168
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发表时间：
2014-07
影响因子：
6.9
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Lou Q;Liu Y;Qi Y;Jiao S;Tian F;Jiang L;Wang Y
通过代谢物分析与转录组测序相结合，提出了一个新的假说来解释蓝色葡萄风信子白色变体缺乏颜色表型。葡萄风信子（Muscari）是一种重要的观赏球根植物，具有独特的蓝色。杏鲍菇的花可以是自然的白色，它提供了一个机会来解开某些生化特征，特别是颜色，背后复杂的代谢网络。构建了M. armeniacum和M.杏属album用于转录组测序。共分离到89926个单转录本，其中143个可以被鉴定为其他物种中颜色相关基因的推定同源物。在综合分析色素化合物与基因表达谱关系的基础上，对M.杏此外，提出了一个新的假说，解释缺乏颜色表型的葡萄风信子花。黄酮醇合成酶（FLS）和二氢黄酮醇4-还原酶（DFR）之间的底物竞争可能导致蓝色色素的消除，而花青素（Cy）合成途径中有限流量的多重分流可能是导致M.白色花颜色变化的最可能原因。杏此外，通过对M. armeniacum及其白色变异株的分离和鉴定，为进一步研究其功能和分子生物学提供了平台。杏
Through a combination of metabolite analysis with transcriptome sequencing, a new hypothesis was proposed to explain the lack of colour phenotype of the white variant of the blue grape hyacinth. Grape hyacinth (Muscari) is an important ornamental bulbous plant with an extraordinary blue colour. Muscari armeniacum, whose flowers can be naturally white, provides an opportunity to unravel the complex metabolic networks underlying certain biochemical traits, especially colour. A blue flower cDNA library of M. armeniacum and a white flower library of M. armeniacum f. album were used for transcriptome sequencing. A total of 89 926 uni-transcripts were isolated, 143 of which could be identified as putative homologues of colour-related genes in other species. Based on a comprehensive analysis relating colour compounds to gene expression profiles, the mechanism of colour biosynthesis was studied in M. armeniacum. Furthermore, a new hypothesis explaining the lack of colour phenotype of the grape hyacinth flower is proposed. Alteration of the substrate competition between flavonol synthase (FLS) and dihydroflavonol 4-reductase (DFR) may lead to elimination of blue pigmentation while the multishunt from the limited flux in the cyanidin (Cy) synthesis pathway seems to be the most likely reason for the colour change in the white flowers of M. armeniacum. Moreover, mass sequence data obtained by the deep sequencing of M. armeniacum and its white variant provided a platform for future function and molecular biological research on M. armeniacum.