PURIFICATION AND COMPLETE AMINO-ACID-SEQUENCE OF ALPHA-HUMAN ATRIAL NATRIURETIC POLYPEPTIDE (ALPHA-HANP)
PURIFICATION AND COMPLETE AMINO-ACID-SEQUENCE OF ALPHA-HUMAN ATRIAL NATRIURETIC POLYPEPTIDE (ALPHA-HANP)
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DOI：
10.1016/0006-291x(84)91077-5
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发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
MATSUO, H
中科院分区：
文献类型：
--
作者：
KANGAWA, K;MATSUO, H
本文采用体外试验法对人心房提取物中钠素因子对鸡直肠收缩性的松弛作用进行了研究。三个不同的组成部分。度量。在粗提物的色谱区发现了一种有效的松弛活性。.alpha。- mw3000道尔顿成分，一种28个氨基酸的肽在纯净状态下被分离出来，当注射到实验大鼠体内时，产生了有效的利尿和利钠活性以及血管松弛活性。因此，一个名称，.alpha。-人心房利钠肽。-hANP)，为多肽。通过微测序和合成，确定了该肽的完整氨基酸序列。
The present survey for natriuretic factors in human atrial extract was performed by using in vitro assay for the relaxant effect on the contractility of chick rectum. Three distinct components (.alpha., .beta. and .gamma.) of a potent relaxant activity were found in the chromatographic regions of the crude extract. As .alpha.-component of MW 3000 daltons, a 28-amino acid peptide was isolated in a pure state and elicited potent diuretic and natriuretic activities as well as vasorelaxant activity, when injected into the assay rats. Accordingly, a name, .alpha.-human atrial natriuretic polypeptide (.alpha.-hANP), for the peptide was proposed. The complete amino acid sequence of the peptide has been established by microsequencing as well as synthesis.