Plant defenses: Chlorogenic acid and polyphenol oxidase enhance toxicity ofBacillus thuringiensis subsp.kurstaki toHeliothis zea
Plant defenses: Chlorogenic acid and polyphenol oxidase enhance toxicity ofBacillus thuringiensis subsp.kurstaki toHeliothis zea
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DOI：
10.1007/bf00994435
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发表时间：
2004
影响因子：
2.3
通讯作者：
C. T. Ludlum;G. Felton;S. Duffey
中科院分区：
文献类型：
--
作者：
C. T. Ludlum;G. Felton;S. Duffey
两种与番茄植株抗性有关的化学物质，绿原酸和多酚氧化酶，已知在受损的植物组织中形成邻苯二酚。据报道，邻苯二酚类化合物可使−NH2和−SH基团的蛋白质和氨基酸烷基化，改变溶解性、消化性，对某些致病病毒来说，还能改变传染性。本文探讨了一种重要的微生物杀虫剂--苏云金芽孢杆菌枯草芽孢杆菌(BTK)对玉米象幼虫的毒性作用及其对苯酚烷基化的影响。当BTK单独与多酚氧化酶孵育时，也出现了类似但不那么戏剧性的结果。消化率实验表明，烷基化促进了体内晶体蛋白的增溶和/或蛋白质分解。我们的结果对BTK与寄主植物抗性和生物防治的兼容性的影响进行了讨论。
Two chemicals implicated in resistance of the tomato plant, chlorogenic acid and polyphenol oxidase, are known to form orthoquinones in damaged plant tissue. Orthoquinones have been reported to alkylate −NH2and −SH groups of proteins and amino acids, altering solubility, digestibility, and, for some pathogenic viruses, infectivity. Here we explore effects of quinone alkylation on toxicity of an important microbial insecticide,Bacillus thuringiensissubsp.kurstaki(BTk), to larvalHeliothis zea.BTk incubated with these phytochemicals and fed to larvae was more toxic than untreated BTk. Similar but less dramatic results arose when BTk was incubated with polyphenol oxidase alone. Digestibility experiments suggest that alkylation enhanced the solubilization and/or proteolysis of crystal protein in vivo. Implications of our results for compatibility of BTk with host-plant resistance and biological control are discussed.