Genetic variants of HvGlb1 in Tibetan annual wild barley and cultivated barley and their correlation with malt quality
Genetic variants of HvGlb1 in Tibetan annual wild barley and cultivated barley and their correlation with malt quality
复制标题
西藏一年生野生大麦和栽培大麦HvGlb1遗传变异及其与麦芽品质的相关性
DOI：
10.1016/j.jcs.2010.09.006
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发表时间：
2011-01-01
影响因子：
3.8
通讯作者：
Zhang, Guoping
中科院分区：
文献类型：
--
作者：
Jin, Xiaoli;Cai, Shengguan;Zhang, Guoping
改良麦芽品质是麦芽大麦育种的重要目标。本试验研究了西藏一年生野生大麦和栽培大麦麦芽品质性状在基因型间的差异，以及编码β-葡聚糖酶同工酶I的HvGlb 1基因变异体的差异。这些基因变异与麦芽品质的相关性分析表明，不同大麦品种间的Kolbach指数、糖化力、粘度和麦芽浸出物4个品质参数存在较大差异。Kolbach指数分别与粘度和麦芽浸出物呈负相关和正相关，而麦芽浸出物与粘度呈负相关。麦芽β-葡聚糖含量是麦芽质量的主要决定因素，与科尔巴赫指数（-0.633）、麦芽浸出物（-0.333）和粘度（0.672）呈显著相关。麦芽β-葡聚糖含量主要受麦芽β-葡聚糖酶活性的控制。相关分析表明，HvGlb 1基因与麦芽β-葡聚糖含量和4个主要麦芽品质参数中的3个（DP除外）相关。此外，我们还发现西藏大麦的HvGlb 1在单倍型上比栽培大麦具有更广泛的多样性，支持西藏是栽培大麦起源中心之一的假设。(C)2010爱思唯尔有限公司版权所有。
Improvement of malt quality is the most important objective in malt barley breeding. The current experiments investigated the variation of malt quality characters among barley genotypes and the difference in genetic variants of HvGlb1, encoding beta-glucanase isoenzyme I, between Tibetan annual wild barley and cultivated barley. The correlation between the gene variants and malt quality showed that there was a large difference in the four malt quality parameters, i.e. Kolbach index, diastatic power (DP), viscosity and malt extract, among the analyzed barley cultivars. Kolbach index was negatively and positively correlated with viscosity and malt extract, respectively, while malt extract was negatively correlated with viscosity. Malt beta-glucan content was a major determinant of malt quality, and was significantly correlated with Kolbach index (-0.633), malt extract (-0.333) and viscosity (0.672). On the other hand, malt beta-glucan content was mainly controlled by malt beta-glucanase activity. The correlation analysis showed that the HvGlb1 gene was correlated with malt beta-glucan content and three of four main malt quality parameters, except DP. In addition, we also found that the HvGlb1 of Tibetan barley had wider diversity in haplotype than that of the cultivated barley, supporting the hypothesis that Tibet is one of the original centers of cultivated barley. (C) 2010 Elsevier Ltd. All rights reserved.