Cyclic AMP and pituitary adenylate cyclase-activating polypeptide (PACAP) prevent programmed cell death of cultured rat cerebellar granule cells
Cyclic AMP and pituitary adenylate cyclase-activating polypeptide (PACAP) prevent programmed cell death of cultured rat cerebellar granule cells
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DOI：
10.1016/s0304-3940(96)12468-x
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发表时间：
1996-03-15
影响因子：
2.5
通讯作者：
Wang, JZ
中科院分区：
文献类型：
--
作者：
Chang, JY;Korolev, VV;Wang, JZ
培养的小脑颗粒细胞经历程序性细胞死亡时，他们被剥夺去极化氯化钾。该研究的结果表明，cAMP类似物环8-（4-氯苯硫基）腺苷-3 '5 '-单磷酸（CPT-cAMP）可以剂量依赖性方式防止细胞死亡，在500 μ M时观察到最大效果。大约70%的细胞可以用该浓度的CPT-cAMP保存至少6天。腺苷酸环化酶激活多肽（PACAP）以时间和剂量依赖性方式增加这些细胞的细胞内cAMP水平。该药物可防止KCl戒断引起的cAMP降低和细胞死亡。这些结果表明，PACAP可以作为一种神经营养因子的小脑颗粒细胞在体内。
Cultured cerebellar granule cells undergo programmed cell death when they are deprived of depolarizing KCl. Results from this study indicate that the cAMP analog cyclic 8-(4-chlorophenylthio) adenosine-3'5'-monophosphate (CPT-cAMP) can prevent the cell death in a dose-dependent manner, with the maximal effect seen at 500 mu M. Approximately 70% of cells can be saved with this concentration of CPT-cAMP for at least 6 days. Pituitary adenylate cyclase-activating polypeptide (PACAP) increased the intracellular cAMP levels of these cells in a time- and dose-dependent manner. This agent can prevent the decrease of cAMP and cell death induced by KCl withdrawal. These results suggest that PACAP could function as a neurotrophic factor for cerebellar granule cells in vivo.