REQUIREMENT FOR PROTEIN-SYNTHESIS IN REGULATION OF PROTEIN BREAKDOWN IN CULTURED HEPATOMA-CELLS
REQUIREMENT FOR PROTEIN-SYNTHESIS IN REGULATION OF PROTEIN BREAKDOWN IN CULTURED HEPATOMA-CELLS
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DOI：
10.1021/bi00694a028
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发表时间：
1975-01-01
期刊：
影响因子：
2.9
通讯作者：
HERSHKO, A
中科院分区：
文献类型：
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作者：
EPSTEIN, D;ELIASBISHKO, S;HERSHKO, A
在培养的肝癌(HTC)细胞中，研究了胰岛素和蛋白质合成抑制剂对标记细胞蛋白降解的作用模式。当缺乏血清(通常存在于培养介质中)时，蛋白质的分解会加速，这种增强可以被胰岛素或放线菌亚胺抑制。一个例外是一类有限的快速翻转的细胞蛋白质，它们的降解不受胰岛素或放线菌亚胺的影响。另一种解释蛋白质合成与蛋白质分解调控关系的假说，即蛋白质合成前体的水平控制，核糖体循环状态的调控，或蛋白质合成产物的需求。蛋白质分解不受氨基酸剥夺的影响，HTC细胞在不同的实验条件下valyl-tRNA水平的测量
The modes of action of insulin and of inhibitors of protein synthesis on the degradation of labeled cellular proteins have been studied in cultured hepatoma (HTC) cells. Protein breakdown is accelerated upon the deprivation of serum (normally present in theculture medium), and this enhancement is inhibited by either insulin or cycloheximide. An exception is a limited class of rapidly turning over cellu-lar proteins, the degradation of which is not influenced by insulin or cycloheximide. Alternative hypothesesto explain the relationship of protein synthesisto the regulation of pro-tein breakdown, viz., control by the levels of precursors of protein synthesis, regulation by thestate of the ribosome cycle, or requirement for a product of protein synthesis, have been examined. Protein breakdown was not influenced by amino acid deprivation, and measurements of valyl-tRNA levels in HTC cells subjected to variousexperimental