CD226 reduces endothelial cell glucose uptake under hyperglycemic conditions with inflammation in type 2 diabetes mellitus.
CD226 reduces endothelial cell glucose uptake under hyperglycemic conditions with inflammation in type 2 diabetes mellitus.
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CD226 在 2 型糖尿病炎症高血糖条件下减少内皮细胞葡萄糖摄取
DOI：
10.18632/oncotarget.7505
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发表时间：
2016-03-15
期刊：
影响因子：
--
通讯作者：
Zhuang R
中科院分区：
文献类型：
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作者：
Zhang Y;Liu T;Chen Y;Dong Z;Zhang J;Sun Y;Jin B;Gao F;Guo S;Zhuang R
CD 226是在免疫和内皮细胞上发现的共刺激粘附分子。在这里，我们评估了CD 226在抑制分离的人脐静脉内皮细胞（HUVEC）和野生型（WT）和CD 226敲除（KO）高脂饮食（HFD）诱导的2型糖尿病（T2 DM）小鼠中葡萄糖摄取的可能作用。在TNF-α存在的高血糖条件下，CD 226表达增加。此外，CD 226敲低改善了内皮细胞中的葡萄糖摄取，并且CD 226 KO小鼠表现出增加的葡萄糖耐量。口服葡萄糖耐量试验（OGTT）后T2 DM患者血浆中可溶性CD 226水平高于空腹条件下。我们的研究结果表明，轻度炎症加上血糖升高增加CD 226表达，导致T2 DM内皮细胞葡萄糖摄取减少。
CD226 is a co-stimulatory adhesion molecule found on immune and endothelial cells. Here, we evaluated a possible role for CD226 in inhibiting glucose uptake in isolated human umbilical vein endothelial cells (HUVECs) and in wild-type (WT) and CD226 knockout (KO) mice with high-fat diet (HFD)-induced type 2 diabetes (T2DM). CD226 expression increased under hyperglycemic conditions in the presence of TNF-α. Furthermore, CD226 knockdown improved glucose uptake in endothelial cells, and CD226 KO mice exhibited increased glucose tolerance. Levels of soluble CD226 in plasma were higher in T2DM patients following an oral glucose tolerance test (OGTT) than under fasting conditions. Our results indicate that low-grade inflammation coupled with elevated blood glucose increases CD226 expression, resulting in decreased endothelial cell glucose uptake in T2DM.