Heat shock-induced translational control of HSP70 and globin synthesis in chicken reticulocytes
Heat shock-induced translational control of HSP70 and globin synthesis in chicken reticulocytes
复制标题
热休克诱导鸡网织红细胞 HSP70 和珠蛋白合成的翻译控制
DOI：
10.1128/mcb.4.11.2437-2448.1984
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发表时间：
1984
影响因子：
5.3
通讯作者：
R. Morimoto
中科院分区：
文献类型：
--
作者：
S. S. Banerji;N. Theodorakis;R. Morimoto
鸡网织红细胞在升高的温度（43至45摄氏度）下孵育导致蛋白质合成模式的快速变化，其特征在于正常蛋白质的合成减少，例如，α和β珠蛋白，以及仅一种热休克蛋白HSP 70的优先和增加的合成。珠蛋白合成的抑制不是由于珠蛋白mRNA的修饰，因为珠蛋白mRNA的水平及其在体外翻译的能力不受影响。网织红细胞中的HSP 70基因在非热休克细胞中转录，但HSP 70直到热休克细胞才被有效翻译。在非热休克的网织红细胞中，HSP 70 mRNA是一种中等丰度的mRNA，含量为球蛋白mRNA水平的1%至2%。热休克后HSP 70的合成迅速增加20倍，但并不伴随着HSP 70基因转录速率的相应增加或HSP 70 mRNA的积累。这些结果提示热休克后网织红细胞HSP 70的合成增加是由于HSP 70 mRNA被优先利用所致。热休克引起的网织红细胞蛋白合成装置的改变是不可逆的。当回到控制温度（37摄氏度），热休克网织红细胞继续合成HSP 70在升高的水平，而珠蛋白的合成继续受到抑制。尽管在非热休克网织红细胞中存在HSP 70 mRNA，我们发现，热休克细胞中HSP 70的优先翻译需要持续转录。用转录抑制剂放线菌素D或5，6-二氯-1-β-D-呋喃核糖基苯并咪唑预孵育网织红细胞可阻断热休克诱导的HSP 70合成。由于HSP 70 mRNA的水平在用放线菌素D处理的细胞中仅略有减少，我们提出了两种可能的机制为HSP 70 mRNA的优先翻译：只翻译新转录的HSP 70 mRNA或需要一个新转录的含RNA的因子。
Incubation of chicken reticulocytes at elevated temperatures (43 to 45 degrees C) resulted in a rapid change in the pattern of protein synthesis, characterized by the decreased synthesis of normal proteins, e.g., alpha and beta globin, and the preferential and increased synthesis of only one heat shock protein, HSP70. The repression of globin synthesis was not due to modifications of globin mRNA because the level of globin mRNA and its ability to be translated in vitro were unaffected. The HSP70 gene in reticulocytes was transcribed in non-heat-shocked cells, yet HSP70 was not efficiently translated until the cells had been heat shocked. In non-heat-shocked reticulocytes, HSP70 mRNA was a moderately abundant mRNA present at 1 to 2% of the level of globin mRNA. The rapid 20-fold increase in the synthesis of HSP70 after heat shock was not accompanied by a corresponding increase in the rate of transcription of the HSP70 gene or accumulation of HSP70 mRNA. These results suggest that the elevated synthesis of HSP70 is due to the preferential utilization of HSP70 mRNA in the heat-shocked reticulocyte. The heat shock-induced alterations in the reticulocyte protein-synthetic apparatus were not reversible. Upon return to control temperatures (37 degrees C), heat-shocked reticulocytes continued to synthesize HSP70 at elevated levels whereas globin synthesis continued to be repressed. Despite the presence of HSP70 mRNA in non-heat-shocked reticulocytes, we found that continued transcription was necessary for the preferential translation of HSP70 in heat-shocked cells. Preincubation of reticulocytes with the transcription inhibitor actinomycin D or 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole blocked the heat shock-induced synthesis of HSP70. Because the level of HSP70 mRNA was only slightly diminished in cells treated with actinomycin D, we suggest two possible mechanisms for the preferential translation of HSP70 mRNA: the translation of only newly transcribed HSP70 mRNA or the requirement of a newly transcribed RNA-containing factor.
mRNA 竞争在调节翻译中的作用：mRNA 歧视起始因素的进一步表征。
DOI：
10.1073/pnas.80.3.663
发表时间：
1983
影响因子：
11.1
作者：
Ray,BK;Brendler,TG;Adya,S;Daniels-McQueen,S;Miller,JK;Hershey,JW;Grifo,JA;Merrick,WC;Thach,RE
通讯作者：
Thach,RE
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Dolan,M;Dodgson,JB;Engel,JD
通讯作者：
Engel,JD