A cost-efficient open source laser engine for microscopy
A cost-efficient open source laser engine for microscopy
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DOI：
10.1101/796482
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发表时间：
2019-10
期刊：
影响因子：
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通讯作者：
Daniel Schröder;Joran Deschamps;Anindita Dasgupta;U. Matti;J. Ries
中科院分区：
文献类型：
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作者：
Daniel Schröder;Joran Deschamps;Anindita Dasgupta;U. Matti;J. Ries
科学级激光器是现代显微镜的昂贵组件。对于高功率应用，如单分子定位显微镜，其价格可能会变得令人望而却步。在这里，我们提出了一种开源的高功率激光引擎，可以以一小部分的成本建造。它使用价格实惠但功能强大的激光二极管，波长为405 nm，488 nm和640 nm，可选561 nm二极管泵浦固态激光器。光通过搅动的多模光纤传输到显微镜，以抑制散斑。我们提供零件清单、CAD文件和详细说明，允许任何研究小组构建自己的激光引擎。
Scientific-grade lasers are costly components of modern microscopes. For high-power applications, such as single-molecule localization microscopy, their price can become prohibitive. Here, we present an open-source high-power laser engine that can be built for a fraction of the cost. It uses affordable, yet powerful laser diodes at wavelengths of 405 nm, 488 nm and 640 nm and optionally a 561 nm diode-pumped solid-state laser. The light is delivered to the microscope via an agitated multimode fiber in order to suppress speckles. We provide the part lists, CAD files and detailed descriptions, allowing any research group to build their own laser engine.