PA0833 Is an OmpA C-Like Protein That Confers Protection Against Pseudomonas aeruginosa Infection.
PA0833 Is an OmpA C-Like Protein That Confers Protection Against Pseudomonas aeruginosa Infection.
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PA0833 是一种 OmpA C 样蛋白，可预防铜绿假单胞菌感染
DOI：
10.3389/fmicb.2018.01062
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发表时间：
2018
影响因子：
5.2
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Yang F;Gu J;Zou J;Lei L;Jing H;Zhang J;Zeng H;Zou Q;Lv F;Zhang J
铜绿假单胞菌是一种可引起高死亡率感染的可怕病原体。由于铜绿假单胞菌能够形成生物被膜并迅速对许多一线抗生素产生耐药性，因此传统的抗生素治疗方案通常难以治愈与铜绿假单胞菌相关的感染。因此，迫切需要新的战略来预防和治疗这种感染。PA0833是一种新鉴定的铜绿假单胞菌保护性抗原，是我们实验室用反向疫苗策略在筛查中鉴定出来的。在本研究中，我们进一步证实了其在小鼠脓毒症和肺炎模型中的保护作用。PA0833免疫诱导了强烈的免疫反应，减少了细菌负荷；减少了病理、炎性细胞因子的表达和炎性细胞的浸润；提高了存活率。此外，通过生物信息学分析和生化特征鉴定，PA0833是一种类似OmpA C的蛋白，与细菌的环境胁迫抗性和毒力有关。这些结果表明，PA0833是一种类似OmpA C的蛋白，能在小鼠体内诱导保护性免疫反应，表明PA0833是一种有希望的疫苗开发抗原。
Pseudomonas aeruginosa is a formidable pathogen that causes infections with high mortality rates. Because of its ability to form biofilms and rapidly acquire resistance to many first-line antibiotics, P. aeruginosa-related infections are typically difficult to cure by traditional antibiotic treatment regimes. Thus, new strategies to prevent and treat such infections are urgently required. PA0833 is a newly identified protective antigen of P. aeruginosa that was identified in a screen using a reverse vaccine strategy in our laboratory. In this study, we further confirmed its protective efficacy in murine sepsis and pneumonia models. Immunization with PA0833 induced strong immune responses and resulted in reduced bacterial loads; decreased pathology, inflammatory cytokine expression and inflammatory cell infiltration; and improved survival. Furthermore, PA0833 was identified as an OmpA C-like protein by bioinformatics analysis and biochemical characterization and shown to contribute to bacterial environmental stress resistance and virulence. These results demonstrate that PA0833 is an OmpA C-like protein that induces a protective immune response in mice, indicating that PA0833 is a promising antigen for vaccine development.