Spatiotemporal patterns of macrophage migration inhibitory factor (Mif) expression in the mouse placenta
Spatiotemporal patterns of macrophage migration inhibitory factor (Mif) expression in the mouse placenta
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DOI：
10.1186/1477-7827-8-95
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发表时间：
2010-08-04
影响因子：
4.4
通讯作者：
Bevilacqua, Estela
中科院分区：
文献类型：
--
作者：
Faria, Miriam R.;Hoshida, Mara S.;Bevilacqua, Estela
背景资料：巨噬细胞移动抑制因子（macrophagemigrationinhibitoryfactor，MIF）具有特殊的促炎作用，影响巨噬细胞和淋巴细胞的功能，对抗糖皮质激素对免疫反应的影响。MIF在人类着床和早期胚胎发育过程中的显著表达也表明该因子在生殖功能中起作用。本研究的总体目标是评估MIF表达的滋养层细胞和胚胎胎盘细胞在小鼠pregnancy.Methods：MIF是immunolocalized在植入部位妊娠天数（GD）7.5，10.5，13.5和17.5。结果：胚胎着床后（gd7.5），滋养层巨细胞对Mif有较强的反应性。在胎盘形成后期（gds 10.5-17.5），Mif似乎集中在交界区和滋养层巨细胞。Mif蛋白表达从gd7.5到10.5（p = 0.005）和从gd7.5到13.5（p = 0.03）显著增加，随着妊娠的进行保持在高浓度。在gd10.5表达量较高，与gd13.5相比差异显著（p = 0.048）和17.5（p = 0.009）.结论：Mif在gd10.5的上调与胎盘呈现其三层组织的阶段一致（巨细胞、海绵滋养层和迷路区），胎儿血液循环开始，uNK细胞的群体在胎盘的母体对应部分达到高比例，提示Mif可能在胎盘形成或在分化的胎盘适应子宫或仍在妊娠免疫调节反应中起作用。此外，它加强了Mif在母体胎儿界面的特定活动的可能性。
Background: Macrophage migration inhibitory factor (MIF) has special pro-inflammatory roles, affecting the functions of macrophages and lymphocytes and counter-regulating the effects of glucocorticoids on the immune response. The conspicuous expression of MIF during human implantation and early embryonic development also suggests this factor acts in reproductive functions. The overall goal of this study was to evaluate Mif expression by trophoblast and embryo placental cells during mouse pregnancy.Methods: Mif was immunolocalized at implantation sites on gestation days (gd) 7.5, 10.5, 13.5 and 17.5. Ectoplacental cones and fetal placentas dissected from the maternal tissues were used for Western blotting and qRT-PCR assays on the same gestation days.Results: During the post-implantation period (gd7.5), trophoblast giant cells showed strong Mif reactivity. In later placentation phases (gds 10.5-17.5), Mif appeared to be concentrated in the junctional zone and trophoblast giant cells. Mif protein expression increased significantly from gd7.5 to 10.5 (p = 0.005) and from gd7.5 to 13.5 (p = 0.03), remaining at high concentration as gestation proceeded. Higher mRNA expression was found on gd10.5 and was significantly different from gd13.5 (p = 0.048) and 17.5 (p = 0.009).Conclusions: The up-regulation of Mif on gd10.5 coincides with the stage in which the placenta assumes its three-layered organization (giant cells, spongiotrophoblast and labyrinth zones), fetal blood circulation begins and population of uNK cells reaches high proportions at the maternal counter part of the placenta, suggesting that Mif may play a role in either the placentation or in the adaptation of the differentiated placenta to the uterus or still in gestational immunomodulatory responses. Moreover, it reinforces the possibility of specific activities for Mif at the maternal fetal interface.