Inadequate protein intake affects skeletal muscle transcript profiles in older humans
Inadequate protein intake affects skeletal muscle transcript profiles in older humans
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DOI：
10.1093/ajcn/85.5.1344
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发表时间：
2007-05-01
影响因子：
7.1
通讯作者：
Campbell, Wayne W.
中科院分区：
文献类型：
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作者：
Thalacker-Mercer, Anna E.;Fleet, James C.;Campbell, Wayne W.
背景：膳食蛋白质摄入不足会引起骨骼肌形态和功能的不良变化。这些变化可能反映在早期改变肌肉信使RNA levels.Objective：本研究评估蛋白质摄入量不足是否差异影响骨骼肌转录本浓度和表达谱在老年人。设计：21名老年男性和女性（年龄55-80岁）消耗控制饮食，提供1.2克蛋白质。kg（-1）。d（-1）（足够的蛋白质），然后随机分为两组。kg（-1）。d（-1）[蛋白质不足（IP）组; n = 11]或1.2 g蛋白质。kg（-1）。d（-1）（对照组; n = 10）持续第二周。从每个阶段结束时获得的禁食状态股外侧肌活检样品中分离RNA，并通过使用微阵列分析测量IP组中的转录水平。结果：方差分析显示，蛋白质摄入不足后，有529个转录本差异表达（P < 0.05）。使用错误发现率（FDR）校正来调整多重比较，我们观察到85个转录本差异表达：54个上调，31个下调。差异表达的转录本在免疫、炎症和应激反应（主要上调）;收缩、运动和发育（上调）;细胞外结缔组织（上调）;能量代谢（下调）;蛋白质合成（下调）和增殖（下调）的功能类别中。饮食相关的差异，9个成绩单的表达进行了交叉验证，通过使用实时polymerase chain reaction.Conclusion：结果文件的骨骼肌转录水平的变化引起的短期蛋白质摄入量不足的老年人，可能会出现不良代谢，功能和结构的事件，包括肌肉萎缩。
Background: Inadequate dietary protein intake causes adverse changes in the morphology and function of skeletal muscle. These changes may be reflected in early alterations in muscle messenger RNA levels.Objective: This study assessed whether inadequate protein intake differentially affects skeletal muscle transcript concentrations and expression profiles in older adults. Design: Twenty-one older men and women (aged 55-80 y) consumed controlled diets that provided 1.2 g protein . kg(-1) . d(-1) (adequate protein) for 1 wk and then were randomly assigned to consume either 0.5 g protein . kg(-1) . d(-1) [inadequate protein (IP) group; n = 11] or 1.2 g protein . kg(-1) . d(-1) (control group; n = 10) for a second week. RNA was isolated from fasting-state vastus lateralis biopsy samples obtained at the end of each period, and transcript levels in the IP group were measured by using microarray anlysis. Changes in selected transcript levels were confirmed by real-time polymerase chain reaction in both groups.Results: Analysis of variance showed 529 differentially expressed transcripts (P < 0.05) after inadequate protein intake. Using the false discovery rate (FDR) correction to adjust for multiple comparisons, we observed that 85 transcripts were differentially expressed: 54 were up-regulated and 31 were down-regulated. The differentially expressed transcripts were in functional classes for immune, inflammatory, and stress responses (predominantly up-regulated); contraction, movement, and development (up-regulated); extracellular connective tissue (up-regulated); energy metabolism (down-regulated); protein synthesis (down-regulated); and proliferation (down-regulated). Diet-related differences in the expression of 9 transcripts were cross-validated by using real-time polymerase chain reaction.Conclusion: The results document changes in skeletal muscle transcript levels induced by short-term inadequate protein intakes in older humans that might precede adverse metabolic, functional, and structural events, including muscle wasting.