Simultaneous Determination of Four Iridoid and Secoiridoid Glycosides and Comparative Analysis of Radix Gentianae Macrophyllae and Their Related Substitutes by HPLC
Simultaneous Determination of Four Iridoid and Secoiridoid Glycosides and Comparative Analysis of Radix Gentianae Macrophyllae and Their Related Substitutes by HPLC
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DOI：
10.1002/pca.1206
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发表时间：
2010-07-01
影响因子：
3.3
通讯作者：
Wang, Zhe-Zhi
中科院分区：
文献类型：
--
作者：
Cao, Xiao-Yan;Wang, Zhe-Zhi
简介-Radix Gentianae Macrophyllae是一种传统的中药，经常被用来消除风湿病和缓解疼痛。在中国药典中，有四种gentiana（G. macrophylla，G。straminea，G。Dahurica和G. crassicaulis）被记录为草药药物（G. officinalis和G. siphonantha），通常用作替代品。目前，不同物种之间的LC指纹比较以及缺乏等效应用的证据。要开发一种HPLC方法，用于同时确定四个虹膜和secoiridoid糖苷的同时测定，并对六种gentiana gentiana种类的六种乙酸糖苷和比较研究。在242 nm.Results上使用0.4％水性磷酸和甲醇使用梯度洗脱 - 提出的方法是精确，准确且敏感的，足以同时评估四种虹膜糖苷（swertiamarin，swertiamarin，gentiopicroside and gentiopicroside and sweroside and Sweroside and s six genea genceenta）的定量评估。即使在同一物种的样品中，这四种标记化合物的内容也彼此不同，六种甲状腺元的LC色谱图显示出很高的相似性。缩写 - LC色谱图和四个真正的gentiana物种的化学组成的紧密相似性解释了其流行的用途，以解释其流行的源自源自中药中的gentix gentix gentix aimaphophyphophape。通过比较四种真正的绅士种类，建议这两个替代品可以用作Radix gentianae Macrophyllae，以减轻资源的稀缺性。版权所有（C）2010 John Wiley＆Sons，Ltd。
Introduction - Radix Gentianae Macrophyllae, a traditional Chinese medicine, has been frequently used to dispel rheumatism and ease pain. There are four species of Gentiana (G. macrophylla, G. straminea, G. dahurica and G. crassicaulis) recorded as herbal drugs in the Chinese Pharmacopoeia and two other Gentiana species (G. officinalis and G. siphonantha) are often used as substitutes. Currently, the LC fingerprint comparison among different species and evidence for the equivalent application of these herbs are lacking.Objective - To develop an HPLC method for the simultaneous determination of four iridoid and secoiridoid glycosides and a comparative study of six species of Gentiana.Methodology - HPLC analysis was performed on a C-18 column (Phenomenex, 150 x 4.6 mm, 5 mu m particle size) with gradient elution using 0.4% aqueous phosphoric acid and methanol at 242 nm.Results - The proposed method was precise, accurate and sensitive enough for simultaneous quantitative evaluation of four iridoid and secoiridoid glycosides (loganic acid, swertiamarin, gentiopicroside and sweroside) in the six species of Gentiona. Contents of the four marker compounds varied from each other even among the samples from the same species and the LC chromatograms of the six species of Gentiana showed high similarities.Conslusion - The close similarity of LC chromatograms and chemical composition of the four genuine Gentiana species explain their popular usage as Radix Gentianae Macrophyllae in Chinese medicine. By comparing the four genuine Gentiana species, it is suggested that the two substitutes could be used as Radix Gentianae Macrophyllae to relieve the scarcity of resources. Copyright (c) 2010 John Wiley & Sons, Ltd.