AMELOGENIN POSTTRANSLATIONAL MODIFICATIONS - CARBOXY-TERMINAL PROCESSING AND THE PHOSPHORYLATION OF BOVINE AND PORCINE TRAP AND LRAP AMELOGENINS
AMELOGENIN POSTTRANSLATIONAL MODIFICATIONS - CARBOXY-TERMINAL PROCESSING AND THE PHOSPHORYLATION OF BOVINE AND PORCINE TRAP AND LRAP AMELOGENINS
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DOI：
10.1006/bbrc.1993.2468
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发表时间：
1993-11-30
影响因子：
3.1
通讯作者：
MORADIANOLDAK, J
中科院分区：
文献类型：
--
作者：
FINCHAM, AG;MORADIANOLDAK, J
TRAP和LRAP釉原蛋白组分通过尺寸排阻和反相HPLC从发育中的牙釉质中分离。猪发育中的牙釉质含有与牛相似的TRAP和LRAP成分。氨基酸组成和质谱分析表明，在这两个物种中，LRAP组分的羧基末端序列比先前报道的牛LRAP长两个残基，并且TRAPs和LRAP都含有单个磷酸化残基。这些釉原蛋白多肽是釉蛋白低分子量组分的主要成分。这两个物种的LRAP序列数据表明，釉原蛋白羧基末端加工的机制可能与以前提出的显着不同。
TRAP and LRAP amelogenin components were isolated by size-exclusion and reversed-phase HPLC from developing dental enamel. Porcine developing enamel contains TRAP and LRAP components analogous to those of bovine. Amino acid composition and mass spectrographic analyses established that, in both species, the carboxy-terminal sequences of the LRAP components are two residues longer than previously reported for bovine LRAP, and that both the TRAPs and LRAPs contained a single phosphorylated residue. These amelogenin polypeptides were the principal components of the enamel protein lower molecular weight fraction. The LRAP sequence data for both species suggests that the mechanism of amelogenin carboxy-terminal processing may differ significantly from that previously suggested.