Expression of Unconventional Myosin VI in Oligodendrocytes
Expression of Unconventional Myosin VI in Oligodendrocytes
复制标题
少突胶质细胞中非常规肌球蛋白 VI 的表达
DOI：
10.1007/s11064-017-2377-7
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发表时间：
2017
期刊：
影响因子：
4.4
通讯作者：
and Yoshihide Yamaguchi
中科院分区：
文献类型：
--
作者：
Reiji Yamazaki;Tomoko Ishibashi;Hiroko Baba;and Yoshihide Yamaguchi
髓鞘是一种特殊的多层结构，参与神经系统的各种功能。少突胶质细胞负责中枢神经系统中的髓鞘形成。马达蛋白在少突胶质细胞谱系的分化和髓鞘形成中起重要作用。最近，我们发现，一个非常规的肌球蛋白，肌球蛋白ID（Myo 1d），在成熟的少突胶质细胞中表达，并需要髓鞘样膜在体外形成。先前，Cahoy等人（J Neurosci 28：264-278，2008）报道了另一种非常规肌球蛋白VI（Myo 6）在分化的少突胶质细胞的转录组数据中上调。然而，目前还不确定Myo 6蛋白是否存在于少突胶质细胞中。在这项研究中，分析Myo 6在少突胶质细胞中的表达，我们对成年正常和铜蛋白诱导的脱髓鞘小鼠的大脑进行免疫荧光分析。Myo 6在小脑和胼胝体的成熟少突胶质细胞和少突胶质祖细胞中表达。为了比较肌球蛋白超家族成员在体外的时间表达模式，使用抗Myo 6、肌球蛋白Va（Myo 5a）或Myo 1d与每个阶段特异性少突胶质细胞标记抗体进行双重免疫染色。在培养的少突胶质细胞中，虽然Myo 1d只在成熟的少突胶质细胞中发现，但Myo 6和Myo 5a信号在从少突胶质细胞祖细胞到成熟的少突胶质细胞的所有分化阶段都被检测到。此外，与Myo 5a类似，Myo 6阳性信号仅限于细胞体和突起。这些结果表明，Myo 6是少突胶质细胞谱系细胞中的非常规肌球蛋白之一，可能在网格蛋白相关的内吞作用中发挥作用。
Myelin is a specialized multilamellar structure involved in various functions of the nervous system. Oligodendrocytes are responsible for myelin formation in the central nervous system. Motor proteins play important roles in differentiation and myelin formation of the oligodendrocyte lineage. Recently, we revealed that one of the unconventional myosins, myosin ID (Myo1d), is expressed in mature oligodendrocytes and is required for myelin-like membrane formation in vitro. Previously, Cahoy et al. (J Neurosci 28:264–278, 2008) reported that another unconventional myosin VI (Myo6) is upregulated in transcriptome data of differentiated oligodendrocytes. However, it is uncertain whether Myo6 protein is present in oligodendrocytes. In this study, to analyze expression of Myo6 in oligodendrocytes, we performed immunofluorescence analysis on brains of adult normal and cuprizone-induced demyelination mice. Myo6 expression was detected in mature oligodendrocytes and oligodendrocyte progenitor cells in the cerebellum and corpus callosum. To compare temporal expression patterns of myosin superfamily members in vitro, double immunostainings using anti-Myo6, myosin Va (Myo5a), or Myo1d with each stage-specific oligodendrocyte marker antibody were performed. In cultured oligodendrocytes, although Myo1d was found only in mature oligodendrocytes, Myo6 and Myo5a signals were detected in all stages of differentiation, from oligodendrocyte progenitor cells to mature oligodendrocytes. Additionally, similar to Myo5a, Myo6-positive signals were confined to the cell body and processes. These results showed that Myo6 is one of the unconventional myosins in oligodendrocyte lineage cells, which could play a role in clathrin-related endocytosis.