Elevated muscle vitamin E does not attenuate eccentric exercise-induced muscle injury.
Elevated muscle vitamin E does not attenuate eccentric exercise-induced muscle injury.
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升高的肌肉维生素 E 不会减轻离心运动引起的肌肉损伤。
DOI：
10.1152/jappl.1992.72.6.2168
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Armstrong,RB
中科院分区：
文献类型：
--
作者：
Warren,JA;Jenkins,RR;Packer,L;Witt,EH;Armstrong,RB
本研究的目的是评估肌肉维生素E含量升高对离心运动引起的骨骼肌损伤的影响。将60只Sprague-Dawley大鼠喂以正常（40 IU维生素E/kg食物）或补充（10，000 IU维生素E/kg食物）饮食5周。比目鱼肌损伤的确定使用几个标准：最大强直力和每平方毫米完整纤维的数量减少，肌肉葡萄糖6-磷酸脱氢酶活性和血浆肌酸激酶活性升高，无论是立即（0小时）或2天（48小时）后下坡行走协议。久坐的动物也进行了测试，但没有运动。肌肉维生素E水平显着升高（约3至4倍），和肌肉的敏感性降低，氧化应激，补充后。然而，维生素E补充剂并没有减轻任何标准的伤害。两组运动后0和48 h的最大强直力下降约20%。在0和48 h时，两组中每平方毫米的完整纤维数量减少约30-35%。在48 h时，两组的葡萄糖6-磷酸脱氢酶活性均升高约50-100%，在0 h时，两组的血浆肌酸激酶活性均升高约2- 2.5倍。这些研究结果不支持自由基损伤的肌肉膜在开始损伤离心运动的主要作用，虽然他们不反驳自由基参与的病因。
The purpose of this study was to evaluate the effect of elevated muscle vitamin E content on skeletal muscle damage from eccentric exercise. Sixty Sprague-Dawley rats were put on a normal (40 IU vitamin E/kg food) or supplemented (10,000 IU vitamin E/kg food) diet for 5 wk. Injury in soleus muscle was determined using several criteria: reductions in maximal tetanic force and number of intact fibers per square millimeter and elevations in muscle glucose 6-phosphate dehydrogenase activity and plasma creatine kinase activity, either immediately (0 h) or 2 days (48 h) after a downhill walking protocol. Sedentary animals were also tested but did not exercise. Muscle vitamin E levels were significantly elevated (approximately 3- to 4-fold), and susceptibility of the muscles to oxidant stress was decreased, after supplementation. However, vitamin E supplementation did not attenuate injury by any of the criteria employed. Maximal tetanic force decreased approximately 20% at 0 and 48 h after exercise in both groups. The number of intact fibers per square millimeter decreased approximately 30–35% in both groups at 0 and 48 h. Glucose 6-phosphate dehydrogenase activity increased approximately 50–100% in both groups at 48 h, and plasma creatine kinase activity was elevated approximately 2- to 2.5-fold at 0 h in both groups. These findings do not support a major role for free radical damage to muscle membranes in the initiation of injury from eccentric exercise, although they do not disprove free radical involvement in the etiology.