Molecular Survey and Genetic Identification of Anaplasma Species in Goats from Central and Southern China
Molecular Survey and Genetic Identification of Anaplasma Species in Goats from Central and Southern China
复制标题
中南山羊无形体的分子调查及遗传鉴定
DOI：
10.1128/aem.06848-11
复制
发表时间：
2012-01-01
影响因子：
4.4
通讯作者：
Yin, Hong
中科院分区：
文献类型：
--
作者：
Liu, Zhijie;Ma, Miling;Yin, Hong
摘要无形体属是一类专性细胞内立克次体病原体，对人类和动物的健康有重要影响。在中国中部和南部，对无形体感染的研究很少。本研究旨在测定绵羊无形体、A. bovis和A.从这些地区的262个山羊现场血液样品中分离嗜吞噬细胞菌。单次感染的平均患病率为100%。ovis、黑腹拟步行虫A. bovis和A.嗜吞噬细胞的阳性率分别为15.3%、16.0%和6.1%。混合感染A. ovis和A.感染以牛为主，感染率为27.1%。混合感染A. ovis和A.嗜吞噬细胞A. bovis和A.嗜吞噬细胞菌为4.2%。三种病原体的合并感染被发现在四个调查的省份，患病率在0和5.3%之间。通过测序证实PCR结果的准确性。对A. bovis和A.嗜吞噬细胞菌证实了这些病原体在调查地点的存在，并表明可能的遗传多样性的A。嗜吞噬细胞菌通过野外动物血液接种，成功地鉴定和观察了A.感染的绵羊单核细胞中的牛病毒。利用msp4基因序列进行系统发育研究。ovis指出，A.北方绵羊的ovis基因型与调查点山羊的基因型不同。
ABSTRACT Anaplasma species are obligate intracellular rickettsial pathogens that impact the health of humans and animals. Few studies have been carried out on Anaplasma infections in central and southern China. This study was conducted to determine the coinfection rates of Anaplasma ovis, A. bovis, and A. phagocytophilum from 262 field blood samples of goats in these regions. The average prevalences of single infection of A. ovis, A. bovis, and A. phagocytophilum were 15.3, 16.0, and 6.1%, respectively. Coinfection of A. ovis and A. bovis was dominant, with an infection rate of 27.1%. Coinfection of A. ovis and A. phagocytophilum was 1.9% and that of A. bovis and A. phagocytophilum was 4.2%. Three-pathogen coinfection was found in three of four investigated provinces with a prevalence between 0 and 5.3%. The accuracy of the PCR results was corroborated by sequencing. Analysis of the 16S rRNA gene sequences of A. bovis and A. phagocytophilum confirmed the presence of these pathogens at the investigated sites and indicated the possible genetic diversity of A. phagocytophilum. Field blood inoculation of experimental animals led to successful identification and observation of the morphological shapes of A. bovis in the infected monocytes of sheep. Phylogenetic study with msp4 sequences of A. ovis indicated that the A. ovis genotypes from sheep in the north differed from the genotypes of goats in the investigated sites.