Pyramiding and evaluation of three dominant brown planthopper resistance genes in the elite indica rice 9311 and its hybrids
Pyramiding and evaluation of three dominant brown planthopper resistance genes in the elite indica rice 9311 and its hybrids
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DOI：
10.1002/ps.3437
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发表时间：
2013-07-01
影响因子：
4.1
通讯作者：
He, Yuqing
中科院分区：
文献类型：
--
作者：
Hu, Jie;Cheng, Mingxing;He, Yuqing
背景 褐飞虱（BPH），Nilaparvata lugens Stal，是水稻产区最具破坏性的害虫。利用标记辅助选择将三个显性褐飞虱抗性基因（Bph14、Bph15、Bph18）整合到优良籼稻 9311 及其杂交种中。根据幼苗和成年水稻的抗性、蜜露重量和褐飞虱的存活率来评估基因有效性。结果所有三个基因均影响褐飞虱的生长发育和抗生素因素，导致幼苗和成虫产生抗性。 Bph15 在赋予 BPH 抗性方面效果最大。结果表明，金字塔基因具有累加效应，基因效应的顺序为14/15/1814/15>15/1815>14/181418>无。金字塔或单基因渗入杂种比传统杂种表现出更大的抗性，尽管杂合基因型的作用比相应的纯合基因型弱。此外，田间试验数据表明，在自然田间条件下，改良9311品系的产量高于或接近于对照。这些改进版本可以立即用于混合动力改进和生产。结论 与对照相比，锥体系和三基因杂种对褐飞虱的抗性最强，且产量没有下降。 (c)2012年化学工业学会
Background Brown planthopper (BPH), Nilaparvata lugens Stal, is the most devastating insect pest in rice-producing areas. Three dominant BPH resistance genes (Bph14, Bph15, Bph18) were pyramided into elite indica rice 9311 and its hybrids using marker-assisted selection. Gene effectiveness was evaluated on the basis of seedling and adult rice resistance, honeydew weight and survival rate of BPH. Results All three genes affected BPH growth and development and antibiotic factors, resulting in both seedling and adult resistance. Bph15 had the greatest effect on conferring resistance to BPH. The results showed an additive effect of pyramiding genes, the order of the gene effect being 14/15/1814/15>15/1815>14/181418>none. The pyramided or single-gene introgression hybrids showed greater resistance than conventional hybrids, although the heterozygous genotypes had weaker effects than the corresponding homozygous genotypes. Furthermore, field trial data demonstrated that yields of improved 9311 lines were higher than or similar to that of the control under natural field conditions. These improved versions can be immediately used in hybrid improvement and production. Conclusion Compared with controls, pyramided lines and hybrids with three genes showed the strongest resistance to BPH, without a yield decrease. (c) 2012 Society of Chemical Industry