Cell micropatterns based on silicone-oil-modified slippery surfaces
Cell micropatterns based on silicone-oil-modified slippery surfaces
复制标题
基于硅油改性光滑表面的细胞微图案
DOI：
10.1039/c6nr07498g
复制
发表时间：
2016
期刊：
影响因子：
6.7
通讯作者：
Wang Shutao
中科院分区：
文献类型：
--
作者：
Shi Wanxin;Xu Tailin;Xu Li-Ping;Chen Yanxia;Wen Yongqiang;Zhang Xueji;Wang Shutao
我们展示了一种简单且环境友好的策略，在由硅油改性的超疏水屏障隔开的纳米树枝状超亲水二氧化硅基底上制造细胞微图案。由于细胞丝状伪足和纳米树突状基底界面之间增强的局部地形相互作用，超亲水性斑点表现出优异的细胞粘附能力，并产生耸人听闻的细胞微图案。相比之下，硅油改性的超疏水屏障的抗粘附性可防止细胞迁移并产生长期的细胞排斥性。这种超亲水点和硅油改性的超疏水屏障非常有助于细胞微图案的形成。此外，不同细胞的共培养可以在硅油修饰的微图案上实现。我们的硅油改性微图案的独特性质为广泛的生物应用提供了相当大的希望，例如基于细胞的生物测定，组织工程，高通量筛选和细胞生物学的基础研究。
We demonstrate a simple and environment-friendly strategy to fabricate cell micropatterns on a nanodendritic superhydrophilic silica substrate separated by silicone-oil-modified superhydrophobic barriers. The superhydrophilic spots exhibit excellent cell adhesion capability due to the enhanced local topographic interaction between cell filopodia and the nanodendritic substrate interface, and result in sensational cell micropatterns. In contrast, the anti-adhesion of silicone-oil-modified superhydrophobic barriers prevents cell migration and results in long-term cell-repellency. Such superhydrophilic spots and silicone-oil-modified superhydrophobic barriers are very helpful for the formation of cell micropatterns. Moreover, co-culture of different cells can be achieved on the silicone-oil-modified micropatterns. The unique properties of our silicone-oil-modified micropatterns hold considerable promise for a wide range of biological applications, such as cell-based bioassays, tissue engineering, high-throughput screening and fundamental studies of cell biology.