Morphometric analysis of smooth muscle in the anterior vaginal wall of women with pelvic organ prolapse
Morphometric analysis of smooth muscle in the anterior vaginal wall of women with pelvic organ prolapse
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DOI：
10.1067/mob.2002.124843
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发表时间：
2002-07-01
影响因子：
9.8
通讯作者：
Word, RA
中科院分区：
文献类型：
--
作者：
Boreham, MK;Wai, CY;Word, RA
目的：本研究的目的是比较正常女性和盆腔器官脱垂女性的阴道前壁平滑肌含量。 研究设计：样本取自 28 名盆腔器官脱垂女性和 12 名对照受试者进行腹部子宫切除术后阴道前壁的顶端。使用平滑肌α-肌动蛋白抗体通过免疫组织化学鉴定阴道前壁的平滑肌细胞。采用形态计量学分析确定阴道前壁组织学横截面肌层中非血管平滑肌的分数面积。结果：与对照组相比，脱垂女性肌层中非血管平滑肌的分数面积显着减少。阴道前壁平滑肌比例的减少与年龄、种族或脱垂阶段无关。在患有脱垂的女性中，未补充雌激素的绝经后女性的阴道平滑肌含量减少最多。绝经前脱垂女性的肌层平滑肌分数面积也显着减少。结论：与正常对照者相比，盆腔器官脱垂女性阴道前壁肌层平滑肌分数显着减少。
OBJECTIVE: The purpose of this study was to compare the smooth muscle content of the anterior vaginal wall in normal women and women with pelvic organ prolapse.STUDY DESIGN: Specimens were taken from the apex of the anterior vaginal cuff after abdominal hysterectomy from 28 women with pelvic organ prolapse and 12 control subjects. Smooth muscle cells of the anterior vaginal wall were identified by immunohistochemistry with antibodies to smooth muscle alpha-actin. Morphometric analysis was used to determine the fractional area of nonvascular smooth muscle in the muscularis in histologic cross-sections of the anterior vaginal wall.RESULTS: The fractional area of nonvascular vaginal smooth muscle in the muscularis of women with prolapse was significantly decreased compared with that of control subjects. This decreased fraction of smooth muscle in the anterior vaginal wall was not related to age, race, or stage of prolapse. In women with prolapse, vaginal smooth muscle content was most diminished in specimens from postmenopausal women with no estrogen replacement. The fractional area of muscularis smooth muscle was also decreased significantly in premenopausal women with prolapse.CONCLUSION: The fraction of smooth muscle in the muscularis of the anterior vaginal wall is significantly decreased in women with pelvic organ prolapse compared with normal control subjects.