Prenatal and postnatal cellularity of the human corneal endothelium. A quantitative histologic study.
Prenatal and postnatal cellularity of the human corneal endothelium. A quantitative histologic study.
复制标题
DOI：
--
复制
发表时间：
1984-03
影响因子：
4.4
通讯作者：
C. Murphy;J. Alvarado;Richard Jusrer;M. Maglio
中科院分区：
文献类型：
--
作者：
C. Murphy;J. Alvarado;Richard Jusrer;M. Maglio
作者用组织学方法研究了56例孕16周到98岁的正常角膜内皮细胞的构成。从每个样本中取10个连续切片，穿过角膜中央6 mm，面积为1.8 mm。对每个切片上的细胞核数量进行计数，并确定每100微米内皮长度的细胞核数量比。这个比率提供了一个测量细胞密度的方法，他们称之为细胞密度。在胎儿期和生命的最初几年，细胞数量以非线性的方式以非常快的速度减少。未观察到可能导致这种明显细胞损失的细胞死亡或坏死。相反，这种细胞结构的快速变化与角膜大小的伴随变化相关。作者的计算表明，细胞分裂可能在胎儿生命的第二个三个月后的内皮形成中起次要作用，因为大多数出生时存在的细胞此时已经存在。在生命的最初几年后，内皮细胞结构的变化率降低，在所检查的近100年生命的其余时间内以线性方式进行。后一种与年龄相关的细胞结构下降可能是由于内皮层每年损失0.56%的细胞，因为角膜在此期间的大小似乎没有变化。对作者数据的统计分析表明，这些结果是非常显著的。
The authors studied the cellularity of the normal corneal endothelium by histologic methods in 56 specimens from 16 weeks of gestation to 98 years of age. Ten step-serial sections were taken from each specimen through the central 6 mm of the cornea, in an area measuring 1.8 mm. The number of nuclei were counted on each section and a ratio of the number of nuclei per 100 micron of endothelial length was determined. This ratio provides a measure of cell density that they call cellularity. There is a decrease in cellularity that proceeds in a nonlinear manner and at a very rapid rate during the prenatal period and for the first few years of life. Cell death or necrosis, which might have contributed to this apparent loss of cells, was not observed. Instead, this rapid change in cellularity is correlated with a concomitant change in corneal size. The authors' calculations show that cell division may play a minor role in the formation of the endothelium after the second trimester of fetal life as most of the cells present by birth already exist by this time. After the first few years of life, the rate of change in endothelial cellularity decreases to proceed in a linear manner for the rest of the near 100 years of life examined. This latter age-related decline in cellularity is probably due to the loss of 0.56% cells per year from the endothelial layer, since the cornea does not appear to change in size during this time. Statistical analysis of the authors' data shows that these results are highly significant.