Synaptonemal Complex Components Are Required for Meiotic Checkpoint Function in Caenorhabditis elegans
Synaptonemal Complex Components Are Required for Meiotic Checkpoint Function in Caenorhabditis elegans
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DOI：
10.1534/genetics.116.191494
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发表时间：
2016-11-01
期刊：
影响因子：
3.3
通讯作者：
Bhalla, Needhi
中科院分区：
文献类型：
--
作者：
Bohr, Tisha;Ashley, Guinevere;Bhalla, Needhi
联会涉及配对同源染色体之间蛋白质结构的组装，联会复合体（SC），并且对于正确的减数分裂染色体分离是必需的。在秀丽隐杆线虫中，突触检查点通过诱导细胞凋亡选择性地去除具有未突触染色体的细胞核。这个检查点依赖于配对中心（PC），促进配对和突触的顺式作用位点。我们假设PC上同源配对的稳定性由该检查点监控。在这里，我们报告说，SC组件SYP-3，HTP-3，HIM-3和HTP-1所需的功能性突触检查点。这些组件的突变不会消除PC功能，表明它们是真正的检查点组件。此外，我们确定突变的背景中，同源配对在PC的不稳定性不相关的突触检查点响应。总之，这些数据表明，除了同源配对，SC组装可以通过突触检查点进行监测。
Synapsis involves the assembly of a proteinaceous structure, the synaptonemal complex (SC), between paired homologous chromosomes, and is essential for proper meiotic chromosome segregation. In Caenorhabditis elegans, the synapsis checkpoint selectively removes nuclei with unsynapsed chromosomes by inducing apoptosis. This checkpoint depends on pairing centers (PCs), cis-acting sites that promote pairing and synapsis. We have hypothesized that the stability of homolog pairing at PCs is monitored by this checkpoint. Here, we report that SC components SYP-3, HTP-3, HIM-3, and HTP-1 are required for a functional synapsis checkpoint. Mutation of these components does not abolish PC function, demonstrating they are bona fide checkpoint components. Further, we identify mutant backgrounds in which the instability of homolog pairing at PCs does not correlate with the synapsis checkpoint response. Altogether, these data suggest that, in addition to homolog pairing, SC assembly may be monitored by the synapsis checkpoint.