TRANSCRIPTS FROM OPPOSITE STRANDS OF GAMMA-SATELLITE DNA ARE DIFFERENTIALLY EXPRESSED DURING MOUSE DEVELOPMENT
TRANSCRIPTS FROM OPPOSITE STRANDS OF GAMMA-SATELLITE DNA ARE DIFFERENTIALLY EXPRESSED DURING MOUSE DEVELOPMENT
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DOI：
10.1007/bf00303248
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发表时间：
1995-02-01
期刊：
影响因子：
2.5
通讯作者：
DOLLE, P
中科院分区：
文献类型：
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作者：
RUDERT, F;BRONNER, S;DOLLE, P
使用体外免疫选择的视黄酸反应元件，我们已经分离出含有被认为是典型的染色体着丝粒的主要（γ）卫星DNA重复序列的小鼠基因组克隆。然后从F9细胞cDNA文库中分离出几个cDNA克隆，发现它们含有234个碱基对共有γ卫星单体的变体。在北方分析中，这些卫星DNA序列主要与来自P19细胞的多聚腺苷酸化RNA中约1.8 kb的RNA种类杂交。这些成绩单强烈抑制视黄酸，和核运行的分析表明，这种抑制，至少部分，介导的转录水平。在HeLa细胞中也检测到卫星转录物，在那里它们同样被类维生素A下调。在特定小鼠组织（如胎儿（但不是胎盘）、成年肝脏和睾丸）的RNA中检测到大小不一的卫星转录物。原位杂交分析表明，卫星转录本是从相反的DNA链产生的，并在发育中的中枢神经系统以及成人肝脏和睾丸的细胞中差异表达。这些数据可能对维甲酸介导的转录调控和着丝粒功能有影响。
Using in vitro immune-selected retinoic acid response elements, we have isolated mouse genomic clones containing major (gamma) satellite DNA repeats that are considered as typical of chromosome centromeres. Several cDNA clones were then isolated from a F9 cell cDNA library and were found to harbor variants of the 234-base pair consensus gamma satellite monomer. In Northern analysis, these satellite DNA sequences hybridized predominantly to an approximate to 1.8-kb RNA species in polyadenylated RNA from P19 cells. These transcripts were strongly repressed by retinoic acid, and nuclear run-on assays revealed that this repression was, at least in part, mediated at the transcriptional level. Satellite transcripts were also detected in HeLa cells, where they were similarly down-regulated by retinoids. Heterogeneously sized satellite transcripts were detected in RNA from specific mouse tissues, such as fetuses (but not placenta), adult liver, and testis. In situ hybridization analysis revealed that satellite transcripts are generated from opposite DNA strands and are differentially expressed in cells of the developing central nervous system as well as in adult liver and testis. These data may have implications on retinoic acid-mediated transcriptional regulation and centromere function.