Synergid cell death in Arabidopsis is triggered following direct interaction with the pollen tube1[W][OA]
Synergid cell death in Arabidopsis is triggered following direct interaction with the pollen tube1[W][OA]
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DOI：
10.1104/pp.107.098236
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发表时间：
2007-08-01
期刊：
影响因子：
7.4
通讯作者：
Drews, Gary N.
中科院分区：
文献类型：
--
作者：
Sandaklie-Nikolova, Linda;Palanivelu, Ravishankar;Drews, Gary N.
在被子植物繁殖过程中，雌性配子体内的两个协同细胞中的一个在受精前发生细胞死亡。花粉管通过生长进入雌配子体的助细胞死亡，并在退化的助细胞质中释放两个精细胞，实现双受精。在拟南芥（拟南芥）和许多其他物种中，协同细胞死亡依赖于授粉。然而，花粉管导致协同细胞死亡的机制尚不清楚。作为理解这一机制的第一步，我们定义了拟南芥花粉管到达雌性配子体与协同细胞死亡之间的时间关系。通过共聚焦激光扫描显微镜、光学显微镜、透射电镜以及在体外对这两个事件的实时观察，我们证明了在花粉管到达雌性配子体之后，在花粉管排出之前，协同细胞死亡就开始了。我们的研究结果支持一个模型，即花粉管-协同细胞接触引发的信号级联诱导拟南芥协同细胞死亡。
During angiosperm reproduction, one of the two synergid cells within the female gametophyte undergoes cell death prior to fertilization. The pollen tube enters the female gametophyte by growing into the synergid cell that undergoes cell death and releases its two sperm cells within the degenerating synergid cytoplasm to effect double fertilization. In Arabidopsis (Arabidopsis thaliana) and many other species, synergid cell death is dependent upon pollination. However, the mechanism by which the pollen tube causes synergid cell death is not understood. As a first step toward understanding this mechanism, we defined the temporal relationship between pollen tube arrival at the female gametophyte and synergid cell death in Arabidopsis. Using confocal laser scanning microscopy, light microscopy, transmission electron microscopy, and real-time observation of these two events in vitro, we demonstrate that synergid cell death initiates after the pollen tube arrives at the female gametophyte but before pollen tube discharge. Our results support a model in which a signaling cascade triggered by pollen tube-synergid cell contact induces synergid cell death in Arabidopsis.