Stanniocalcin, a Potential Ovarian Angiogenesis Regulator, Does Not Affect Endothelial Cell Apoptosis
Stanniocalcin, a Potential Ovarian Angiogenesis Regulator, Does Not Affect Endothelial Cell Apoptosis
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DOI：
10.1111/j.1749-6632.2009.04680.x
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发表时间：
2009-01-01
期刊：
影响因子：
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通讯作者：
Grasselli, Francesca
中科院分区：
文献类型：
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作者：
Basini, Giuseppina;Bussolati, Simona;Grasselli, Francesca
卵泡是研究血管生成过程的一个重要模型。事实上，卵泡发育与广泛的新生血管形成有关，而生理性血管生成是卵泡发生所必需的。然而，虽然触发血管生成事件的主要因素已经被彻底研究，现在相对较好地定义，有关参与调节和/或阻止新血管形成的分子事件的信息仍然很少。因此，本研究重点关注斯钙素（STC）（一种生物学功能尚不清楚的蛋白质）在滤泡血管生成控制机制中的潜在参与。我们评估了5和50 ng/mL STC对猪颗粒细胞产生主要促血管生成因子血管内皮生长因子（VEGF）的影响。此外，STC对猪主动脉内皮细胞的细胞活力和半胱天冬酶-3和-7活性的调节的影响也进行了检查。STC显著抑制颗粒细胞VEGF的生成（P < 0.001）。STC可显著提高内皮细胞活力（P < 0.001），降低caspase活性（P < 0.01）。这些数据表明，STC不是血管内皮细胞的血管抑制，虽然它可以潜在地调节局部血管生成作用在颗粒细胞水平。
The ovarian follicle represents an outstanding model for the investigation of the angiogenic process. In fact, follicular development is associated with an extensive neovascularization, and physiological angiogenesis is necessary for folliculogenesis. However, although the major factors triggering the angiogenic events have been thoroughly investigated and are now relatively well defined, information about the molecular events involved in the modulation and/or arrest of neovascularization is still scarce. Therefore, this research focused on the potential involvement of stanniocalcin (STC), a protein whose biological function is still unclear, in the control mechanisms of follicular angiogenesis. We evaluated the effect of 5 and 50 ng/mL STC on the production of the main proangiogenic factor, vascular endothelial growth factor (VEGF), by swine granulosa cells. Moreover, STC's effect on cell viability and the modulation of caspase-3 and -7 activities in swine aortic endothelial cells were also examined. Granulosa cell VEGF production was significantly (P < 0.001) inhibited by STC. Endothelial cell viability was significantly (P < 0.001) increased, whereas caspase activities were reduced (P < 0.01) by STC. These data indicate that STC is not angiosuppressive for endothelial cells, although it could potentially modulate local angiogenesis acting at the granulosa cell level.