Development of human mast cells from umbilical cord blood cells by recombinant human and murine c-kit ligand.
Development of human mast cells from umbilical cord blood cells by recombinant human and murine c-kit ligand.
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DOI：
10.1073/pnas.90.2.735
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发表时间：
1993-01
影响因子：
11.1
通讯作者：
H. Mitsui;T. Furitsu;A. Dvorak;A. Irani;L. Schwartz;Naoki Inagaki;M. Takei;K. Ishizaka;K. Zsebo;S. Gillis;T. Ishizaka
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文献类型：
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作者：
H. Mitsui;T. Furitsu;A. Dvorak;A. Irani;L. Schwartz;Naoki Inagaki;M. Takei;K. Ishizaka;K. Zsebo;S. Gillis;T. Ishizaka
人和小鼠c-kit配体在脐带血单核细胞的长期培养中诱导人肥大细胞分化。BALB/3T3成纤维细胞条件培养基中人肥大细胞的生长因子活性来源于小鼠c-kit配体。重组c-kit配体诱导肥大细胞祖细胞早期分化和增殖。然而，c-kit配体在脐带血细胞培养中明显的选择性肥大细胞生长主要是由于细胞因子选择性维持未成熟肥大细胞的存活。电镜分析表明，c-kit配体培养的人肥大细胞与肺和肠粘膜的人肥大细胞相似，而脐带血细胞与Swiss albino/3T3成纤维细胞共培养的人肥大细胞与皮肤肥大细胞相似。c-kit配体培养的大多数肥大细胞颗粒中只含有胰蛋白酶，而共培养的肥大细胞颗粒中同时含有胰蛋白酶和乳糜酶，这一事实支持了这一结论。c-kit配体培养的肥大细胞在培养14周后仍未成熟。然而，这些细胞表达Fc epsilon RI，并且可以被人IgE致敏，以抗IgE诱导的组胺、前列腺素D2和白三烯C4的释放。
Both human and mouse c-kit ligand induced differentiation of human mast cells in a long-term culture of the mononuclear cells of umbilical cord blood. Growth factor activity for human mast cells present in conditioned medium of BALB/3T3 fibroblasts was due to mouse c-kit ligand. Recombinant c-kit ligand induced differentiation and proliferation of mast cell progenitors in early stages of culture. However, apparent selective growth of mast cells by c-kit ligand in cord blood cell cultures is mainly due to the effect of the cytokine to selectively maintain survival of immature mast cells. Electron microscopic analysis indicated that human mast cells developed by c-kit ligand were similar to human mast cells in the lung and gut mucosa, while those developed in coculture of cord blood cells with Swiss albino/3T3 fibroblasts were similar to skin mast cells. This conclusion was supported by the fact that the majority of mast cells developed by c-kit ligand contained only tryptase in their granules, whereas those developed in the cocultures contained both tryptase and chymase. It was also found that mast cells developed by c-kit ligand were immature even after culture for 14 weeks. Nevertheless, these cells express Fc epsilon RI, and could be sensitized with human IgE for anti-IgE-induced release of histamine, prostaglandin D2, and leukotriene C4.