GRADIENT DESIGN TO OPTIMIZE RATE ZONAL SEPARATIONS
GRADIENT DESIGN TO OPTIMIZE RATE ZONAL SEPARATIONS
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DOI：
10.1016/0003-2697(73)90203-0
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发表时间：
1973-01-01
影响因子：
2.9
通讯作者：
COTMAN, CW
中科院分区：
文献类型：
--
作者：
CHURCHIL.L;BANKER, G;COTMAN, CW
通过分析颗粒在线性蔗糖梯度中的沉降，提出了一种最大化分辨率的梯度设计方法。我们的分析建立了率分离的基本原则。这些原则可以帮助成功设计制备离心程序。在均匀介质或低密度的非常浅的梯度中，速率分离总是最佳的。在均匀介质中，仅沉降系数不同的颗粒的分辨率由它们的沉降系数之比决定。除非仔细选择条件，否则沉降性质彼此相反的颗粒在某些条件下不能分离或几乎不能分离。密度比沉降系数相差更大的颗粒群，显然按速率比按等密度带分离得更好。在粘度随密度增加而降低的梯度类型中，梯度中的速率分离得到显著改善。
An approach to the design of gradients which maximize resolution is developed by analyzing the sedimentation of particles in linear sucrose gradients. Our analysis establishes the fundamental principles of rate separations. These principles can assist in the successful design of preparative centrifugation procedures. Rate separations are always optimal in homogeneous media or very shallow gradients of low density. In homogeneous media, resolution of particles which differ only in sedimentation coefficients is determined by the ratio of their sedimentation coefficients. Particles whose sedimentation properties oppose each other can, under certain conditions, not separate or barely separate unless conditions are carefully selected. Particle populations which differ more in density than in sedimentation coefficients clearly separate better by rate than by isopycnic banding. Rate separations in gradients are considerably improved in a type of gradient where the viscosity decreased as the density increased.