Leveling response factors in the electrospray ionization process using a heated capillary interface.
Leveling response factors in the electrospray ionization process using a heated capillary interface.
复制标题
使用加热毛细管接口平衡电喷雾电离过程中的响应因子。
DOI：
10.1016/j.jasms.2005.02.002
复制
发表时间：
2005
影响因子：
3.2
通讯作者：
Burke,MichaelJ
中科院分区：
文献类型：
--
作者：
Frahm,JenniferL;Muddiman,DavidC;Burke,MichaelJ
一些研究人员观察到变性 DNA 互补链的电喷雾反应存在差异，这是由于两条链之间的疏水性差异所致。疏水性物质越多，离子丰度越高。加热电喷雾源的实施使我们能够“平衡”具有不同疏水性的两条等摩尔互补链的电喷雾响应。随着温度升高，疏水性较低的非编码链与疏水性较高的编码链的离子丰度之比接近一致。此外，加热的电喷雾源用于使含有 7-脱氮嘌呤的扩增子变性，这可用于促进质谱测序。
Several investigators have observed a discrepancy in electrospray response of complementary strands from denatured DNA, which has been attributed to the difference in hydrophobicity between the two strands; the more hydrophobic species tend to have higher ion abundances. The implementation of a heated electrospray source has allowed us to “level” the electrospray response for two equimolar complementary strands with different hydrophobicities. As the temperature was increased, the ratio of ion abundances of the less hydrophobic noncoding strand to the more hydrophobic coding strand approached unity. Furthermore, the heated electrospray source was used to denature amplicons containing 7-deaza purines, which can be used to facilitate sequencing by mass spectrometry.