Contrasting effects of lactoferrin on human lymphocyte and monocyte natural killer activity and antibody-dependent cell-mediated cytotoxicity.
Contrasting effects of lactoferrin on human lymphocyte and monocyte natural killer activity and antibody-dependent cell-mediated cytotoxicity.
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DOI：
10.4049/jimmunol.129.6.2519
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发表时间：
1982-12
影响因子：
4.4
通讯作者：
K. Nishiya;D. Horwitz
中科院分区：
文献类型：
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作者：
K. Nishiya;D. Horwitz
铁和铁结合蛋白乳铁蛋白 (LF) 对自然杀伤 (NK) 和抗体依赖性细胞毒性 (ADCC) 有显着影响。在短期铬释放测定中，将人贴壁和非贴壁血液单核细胞与 K562 细胞和抗体致敏的 Chang 细胞一起孵育。柠檬酸铁(10(-3)M)抑制贴壁和非贴壁NK细胞活性，但对ADCC没有影响。 LF 显着影响贴壁单核细胞的细胞毒性，但对非贴壁淋巴细胞没有影响。 LF 以剂量依赖性方式增强 NK，但相似的浓度反而抑制 ADCC。 LF 直接作用于贴壁效应细胞，因为细胞预处理 30 分钟足以增强效果。 Fe 饱和 LF 以及不饱和蛋白增强贴壁细胞 NK。所有测试浓度的转铁蛋白均不会改变 NK 活性。这些研究表明，除了之前描述的以外，铁还对免疫功能具有抑制作用，并揭示了 LF 的新调节作用。 LF 对贴壁细胞的选择性作用进一步证明单核细胞，至少在贴壁状态下，可以具有有效的 NK 活性。 LF 对 NK 和 ADCC 的相反作用尚无法解释，可作为定义相关机制的探针。
Iron and the iron-binding protein lactoferrin (LF) have significant effects on natural killer (NK) and antibody-dependent cellular cytotoxicity (ADCC). Human adherent and nonadherent blood mononuclear cells were incubated with K562 cells and antibody-sensitized Chang cells in short-term chromium-release assays. Ferric citrate (10(-3) M) inhibited both adherent and nonadherent NK cell activity, but had no effect upon ADCC. LF markedly affected adherent monocyte cytotoxicity, but had no effect on nonadherent lymphocytes. LF enhanced NK in a dose-dependent manner, but similar concentrations paradoxically inhibited ADCC. LF acted directly upon the adherent effector cell because pretreatment of cells for 30 min was sufficient for enhancement. Fe-saturated LF as well as the unsaturated protein enhanced adherent cell NK. Transferrin in all concentrations tested did not alter NK activity. These studies show inhibitory effects of iron on immune function in addition to those previously described and reveal a new regulatory role for LF. The selective effect of LF on adherent cells provides further evidence that monocytes, at least in the adherent state, can have potent NK activity. The opposite effects of LF on NK and ADCC are unexplained and may serve as a probe to define the mechanisms involved.