Strong increase in the autofluorescence of cells signals struggle for survival
Strong increase in the autofluorescence of cells signals struggle for survival
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DOI：
10.1038/s41598-018-30623-2
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发表时间：
2018-08-14
影响因子：
4.6
通讯作者：
Matic, Ivan
中科院分区：
文献类型：
--
作者：
Surre, Jeremy;Saint-Ruf, Claude;Matic, Ivan
原核和真核细胞由于存在荧光的细胞结构成分和代谢产物而显示出一种固有的自然荧光。因此，细胞自身荧光(AF)预计会随着细胞的代谢状态而变化。我们研究了暴露在不同应激源下如何改变大肠杆菌细胞的AF。我们观察到杀菌处理增加了绿色细胞的房颤，并且需要从头合成蛋白质来增加所观察到的房颤。激发和发射光谱以及黄素生物合成途径中基因表达的增加，强烈表明黄素是导致房颤增加的主要因素。编码多种黄素蛋白的基因表达增加，这些基因参与能量产生和ROS解毒，表明了一种应对严重压力的细胞策略。观察到的应激下AF的增加是一种进化保守的现象，因为它不仅发生在不同细菌物种的细胞中，而且也发生在酵母和人类细胞中。
Prokaryotic and eukaryotic cells exhibit an intrinsic natural fluorescence due to the presence of fluorescent cellular structural components and metabolites. Therefore, cellular autofluorescence (AF) is expected to vary with the metabolic states of cells. We examined how exposure to the different stressors changes the AF of Escherichia coli cells. We observed that bactericidal treatments increased green cellular AF, and that de novo protein synthesis was required for the observed AF increase. Excitation and emission spectra and increased expression of the genes from the flavin biosynthesis pathway, strongly suggested that flavins are major contributors to the increased AF. An increased expression of genes encoding diverse flavoproteins which are involved in energy production and ROS detoxification, indicates a cellular strategy to cope with severe stresses. An observed increase in AF under stress is an evolutionary conserved phenomenon as it occurs not only in cells from different bacterial species, but also in yeast and human cells.