Steroid hormone 20-hydroxyecdysone regulation of the very-high-density lipoprotein (VHDL) receptor phosphorylation for VHDL uptake
Steroid hormone 20-hydroxyecdysone regulation of the very-high-density lipoprotein (VHDL) receptor phosphorylation for VHDL uptake
复制标题
类固醇激素 20-羟基蜕皮酮调节极高密度脂蛋白 (VHDL) 受体磷酸化以促进 VHDL 摄取
DOI：
10.1016/j.ibmb.2013.02.001
复制
发表时间：
2013
影响因子：
3.8
通讯作者：
Zhao Xiao-Fan
中科院分区：
文献类型：
--
作者：
Dong Du-Juan;Liu Wen;Cai Mei-Juan;Wang Jin-Xing;Zhao Xiao-Fan
在全变态昆虫变态阶段，合成大量成虫蛋白质所需的生物合成前体是从选择性吸收贮藏蛋白中获得的。极高密度脂蛋白（VHDL）是一种非六聚体储存蛋白，通过HDL受体（VHDL-R）介导的内吞作用被脂肪体从血淋巴中消耗。然而，VHDL-R摄取VHDL的机制仍不清楚。在这项研究中，从棉铃虫的VHDL-R被发现参与20 E调节的VHDL摄取通过调节类固醇激素20-羟基蜕皮激素（20 E）。游走期VHDL-R主要在脂肪体和珠被中表达。20 E通过蜕皮激素受体（EcRB 1）和超气门（USP 1）上调VHDL-R的转录。此外，20 E通过蛋白激酶C刺激VHDL-R的磷酸化以进行配体结合。VHDL-R基因敲低可抑制幼虫向成体的发育。这些数据表明，20 E调节VHDL-R的转录和翻译后水平的VHDL吸收。
During the metamorphic stage of holometabolous insects, the biosynthetic precursors needed for the synthesis of a large number of adult proteins are acquired from the selective absorption of storage proteins. The very-high-density lipoprotein (VHDL), a non-hexameric storage protein, is consumed by the fat body from the hemolymph through VHDL receptor (VHDL-R)-mediated endocytosis. However, the mechanism of the uptake of VHDL by a VHDL-R remains unclear. In this study, a VHDL-R from Helicoverpa armigera was found to be involved in 20E-regulated VHDL uptake through the regulation of steroid hormone 20-hydroxyecdysone (20E). The transcripts of VHDL-R were detected mainly in the fat body and integument during the wandering stage. The transcription of VHDL-R was upregulated by 20E through the ecdysteroid receptor (EcRB1) and Ultraspiracle (USP1). In addition, 20E stimulates the phosphorylation of VHDL-R through protein kinase C for ligand binding. VHDL-R knockdown in larvae results the inhibition of development to adulthood. These data imply that 20E regulates VHDL-R on both transcriptional and posttranslational levels for VHDL absorption.