Image analysis and mathematical correlation between cell number and diameter of colonies in soft agar as a measurement of growth in soft agar.
Image analysis and mathematical correlation between cell number and diameter of colonies in soft agar as a measurement of growth in soft agar.
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软琼脂中细胞数量和菌落直径之间的图像分析和数学相关性作为软琼脂生长的测量。
DOI：
10.1002/tcm.1770100408
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Milo,GE
中科院分区：
文献类型：
--
作者：
Lewis,JC;Milo,GE
使用自动图像分析测量殖民地直径，并估计在非贴壁生长（AIG）条件下菌落中的细胞数量。直径≤ 60 μm的集落中AIG细胞数量的计算值与自动或手动细胞计数获得的实际值明显不同。与显示AIG的动物细胞一样，这些显示锚定独立性的集落可以重复计数。然而，表达AIG的集落中的细胞数（N）直接随集落直径（d）的变化而变化的假设是不正确的。无论是在一定直径的集落中手动计数细胞数，还是任意使用直径函数≤ 60 μm作为线性距离，表明集落中存在50个或更多细胞，每个程序都有其自身的内置偏倚。在需要定量数据的程序中，最可靠的程序是用菌落中的细胞数标准化菌落直径值。
Automated image analysis was used to measure the colony diameter and estimate the cell number in colonies under conditions for anchorage‐independent growth (AIG). The calculated values for the number of AIG cells in a colony of a diameter ⩾ 60 μm were decidedly different from the actual values obtained by either automated or manual cell counting. Like animal cells that exhibit AIG, these colonies demonstrating anchorage independence can be enumerated reproducibly. However, the assumption is incorrect that the number of cells (N) in a colony expressing AIG varies directly with a change in the diameter of a colony (d). Whether one counts the number of cells manually in a colony of a definite diameter or arbitrarily uses a diameter function of ⩾ 60 μm as a linear distance that indicates the presence of 50 or more cells in a colony, each procedure has its own built‐in bias. In procedures that require quantitative data, the most reliable procedure is to standardize the values for diameter of colonies with cell numbers in colonies.