TANDEMLY REPEATED EXONS ENCODE 81-BASE REPEATS IN MULTIPLE, DEVELOPMENTALLY REGULATED SCHISTOSOMA-MANSONI TRANSCRIPTS
TANDEMLY REPEATED EXONS ENCODE 81-BASE REPEATS IN MULTIPLE, DEVELOPMENTALLY REGULATED SCHISTOSOMA-MANSONI TRANSCRIPTS
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DOI：
10.1128/mcb.8.11.4745
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发表时间：
1988-11-01
影响因子：
5.3
通讯作者：
ROTTMAN, FM
中科院分区：
文献类型：
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作者：
DAVIS, RE;DAVIS, AH;ROTTMAN, FM
曼氏血吸虫成虫cDNA克隆10-3编码一种被感染者血清识别的抗原。我们表征了与10-3 cDNA同源的多个发育调节转录物和编码这些转录物的复杂基因组位点的部分。大约950，870和780个核苷酸的转录本在成人中表达，而只有780个核苷酸的转录本在幼虫阶段观察到。这些成绩单是高度相似的，含有可变数量的相同的81个碱基的直接串联重复。尽管在所有转录物中重复元件的序列和它们的3“的序列是相同的，但重复元件的5”序列表现出变异，包括27个碱基的插入、转录的替代起始位点和替代的5“外显子使用。这些成绩单似乎是由小外显子的发育控制的选择性剪接和使用的选择性转录起始位点从一个或两个复杂的基因座的至少40个酶对。转录本中每个81个碱基的重复序列由三个分散的27个碱基对的外显子编码。这些27个碱基对的外显子包含在高度保守的，重复的3-内切酶对基因组串联阵列中。
The adult Schistosoma mansoni cDNA clone 10-3 encodes an antigen that is recognized by sera from infected humans. We characterized multiple developmentally regulated transcripts homologous to the 10-3 cDNA and portions of the complex genomic loci encoding those transcripts. Transcripts of approximately 950, 870, and 780 nucleotides were expressed in adults, whereas only the 780-nucleotide transcript was observed in the larval stage. These transcripts were highly similar, containing variable numbers of identical direct tandem repeats of 81 bases. Although the sequence of the repeating elements and sequences of 3'' to them were identical in all the transcripts, sequences 5'' of the repeating elements exhibited variations, including a 27-base insertion, alternative start sites for transcription, and alternate 5'' exon usage. These transcripts appeared to be derived in part by the developmentally controlled alternative splicing of small exons and the use of alternative transcription initiation sites from the one or two complex loci of at least 40 kilobase pairs. Each 81-base repeat in the transcripts was encoded by three dispersed 27-base-pair exons. These 27-base-pair exons were contained within highly conserved, reiterated 3-kilobase-pair genomic tandem arrays.