Enzyme Multilayer-Modified Biosensors. Use of Streptavidin and Deglycosylated Avidin for Constructing Glucose Oxidase and Lactate Oxidase Multilayers
Enzyme Multilayer-Modified Biosensors. Use of Streptavidin and Deglycosylated Avidin for Constructing Glucose Oxidase and Lactate Oxidase Multilayers
复制标题
酶多层修饰生物传感器。
DOI：
10.2116/analsci.13.859
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发表时间：
1997
影响因子：
1.6
通讯作者：
Hiroki Takeshita
中科院分区：
文献类型：
--
作者：
J. Anzai;Yuka Kobayashi;Hiroki Takeshita
我们已经报道过，通过在电极表面交替沉积亲和素和生物素标记的酶，可以在蛋白质多层层的层层结构中构建酶（图1）。3由此制备的酶多层修饰电极具有酶生物传感器的功能，并且可以通过调节电极上酶层的数量来控制其性能特征。基于这种技术，目前已制备出葡萄糖、乳酸和酒精传感器。多层酶的形成依赖于生物素标记酶中亲和素和生物素残基之间的非共价和强结合（结合常数：约10 ' M-'），4预计多层酶也可以使用同源酶来构建
We have reported that an enzyme can be built in a layer-by-layer structure of protein multilayers on an electrode surface, by depositing avidin and biotin-labeled enzyme alternately (Fig. 1).1 3 The enzyme multilayermodified electrodes thus prepared function as enzyme biosensors and their performance characteristics can be controlled by regulating the number of the enzyme layers on the electrode. Based on this technique, glucose', lactate2, and alcohol sensors3 have been prepared so far. The formation of the enzyme multilayers relies on a noncovalent and strong binding between avidin and biotin residues in the biotin-labeled enzymes (binding constant: ca. 1O's M-'),4 It is anticipated that the enzyme multilayers can be constructed also using homologous