Calcium-dependent fluorescence lifetimes of Indo-1 for one- and two-photon excitation of fluorescence.
Calcium-dependent fluorescence lifetimes of Indo-1 for one- and two-photon excitation of fluorescence.
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Indo-1 在单光子和双光子激发荧光时的钙依赖性荧光寿命。
DOI：
10.1111/j.1751-1097.1993.tb09571.x
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发表时间：
1993
影响因子：
3.3
通讯作者：
Lakowicz,JR
中科院分区：
文献类型：
--
作者：
Szmacinski,H;Gryczynski,I;Lakowicz,JR
我们表征了 Indo-1 的荧光强度衰减，它通常用作发射波长比率钙探针。 Indo-1 发射的长波长侧的表观寿命取决于 Ca2+。这种长波长发射显示了激发态反应的特征，即多指数分析中的负指前分量。 Indo-1 的发射光谱和寿命似乎分别在 351 mn 和 702 mn 处的单光子和双光子激发下相同，这表明 Indo-1 的无钙和钙结合形式的相对单光子和双光子横截面相似。此外，Indo-1 的双光子截面相对较高，对于无钙和钙结合形式，在 690 nm 处约为 4 × 10−49cm4s/光子分子。因此，Indo-1 可用于基于单光子或双光子激发的钙成像，使用发射波长比或寿命成像方法。
We characterized the fluorescence intensity decays of Indo‐1, which is commonly used as an emission wavelength‐ratiometric calcium probe. The apparent lifetime of the long‐wavelength side of the emission of Indo‐1 is dependent on Ca2+. This long‐wavelength emission displays the characteristics of an excited‐state reaction, that is, a negative preexponential component in thc multiexponential analysis. The emission spectra and lifetime of Indo‐1 appear to be identical for one‐photon and two‐photon excitation at 351 and 702 mn, respectively, suggesting that the relative one‐ and two‐photon cross sections are similar for the calcium‐free and calcium‐bound forms of Indo‐1. Also, the two‐photon cross section of Indo‐1 is relatively high, about 4 × 10−49cm4s/photon molecule at 690 nm for both the calcium‐free and calcium‐bound forms. Hence, Indo‐1 can be used for calcium imaging based on one‐ or two‐photon excitation, using either emission wavelength ratios or lifetime imaging methods.