Regulation of osteoclastogenesis by ganoderic acid DM isolated from Ganoderma lucidum
Regulation of osteoclastogenesis by ganoderic acid DM isolated from Ganoderma lucidum
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DOI：
10.1016/j.ejphar.2008.11.005
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发表时间：
2009-01-05
影响因子：
5
通讯作者：
Kondo, Ryuichiro
中科院分区：
文献类型：
--
作者：
Miyamoto, Ichiko;Liu, Jie;Kondo, Ryuichiro
本实验观察了灵芝乙醇提取物对去势雌性SD大鼠骨密度下降的预防作用。结果表明，G.与Ovx大鼠相比，lucidum治疗的Ovx大鼠显示出改善的骨密度。研究了G.灵芝对骨髓细胞和RAW 264细胞D-克隆（RAW-D）的骨细胞分化的影响。分化，响应于NF-κ B配体的受体激活剂（RANKL）和肿瘤坏死因子（TNF-α），被G.灵芝和灵芝酸DM，其通过生物测定引导的分级分离作为活性化合物之一。灵芝酸DM尤其抑制c-Fos和活化T细胞核因子cl（NFATc 1）的表达。这种抑制导致树突状细胞特异性跨膜蛋白（DC-STAMP）表达的抑制，并减少破骨细胞融合。(C)2008年由Elsevier B. V.出版。
The preventative effects of the ethanol extracts of Ganoderma lucidum against the ovariectomized (Ovx)induced deterioration of bone density in 11-week-old female Sprague Dawley (SD) rats were investigated. The results showed that the G. lucidum-treated Ovx rats showed improved bone density compared with the Ovx rats. We studied the effects of G. lucidum on osteoclastic differentiation using bone marrow cells and RAW 264 cell D-clone (RAW-D). Differentiation, in response to receptor activator of NF-KB ligand (RANKL) and a tumor necrosis factor at (TNF-alpha), was inhibited by the ethanol extracts of G. lucidum and ganoderic acid DM which was isolated as one of the active compounds by bioassay-guided fractionation. Ganoderic acid DM especially suppresses the expression of c-Fos and nuclear factor of activated T cells cl (NFATc1). This suppression leads to the inhibition of dendritic cell-specific transmembrane protein (DC-STAMP) expression and reduces osteoclast fusion. (C) 2008 Published by Elsevier B.V.