Electron transfer between flavodoxin semiquinone and c-type cytochromes: correlations between electrostatically corrected rate constants, redox potentials, and surface topologies.
Electron transfer between flavodoxin semiquinone and c-type cytochromes: correlations between electrostatically corrected rate constants, redox potentials, and surface topologies.
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黄素氧还蛋白半醌和 c 型细胞色素之间的电子转移：静电校正速率常数、氧化还原电位和表面拓扑之间的相关性。
DOI：
10.1021/bi00321a009
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Cusanovich,MA
中科院分区：
文献类型：
--
作者：
Tollin,G;Cheddar,G;Watkins,JA;Meyer,TE;Cusanovich,MA
材料和方法如前所述，通过使用停流分光光度法测量巴氏梭菌黄素氧还蛋白半醌和各种氧化同源细胞色素之间的电子转移速率常数（Jung & Tollin，1981；Simondson 等人，1982；Simondsen & Tollin，1983）。所使用的各种细胞色素的来源如早期出版物中所指定（Meyer 等人，1983）。通过添加适量的 NaCl 来改变离子强度。正如马细胞色素 c 所发现的那样（Simondsen 等人，1982），黄素氧还蛋白半醌与此处使用的其他细胞色素的反应是双相的。与之前的工作一样，所有动力学数据都针对这种双相性进行了校正，因此下面报告的速率常数仅指反应的快相，通常占总反应的 70-80%。
Materials and MethodsRate constants for electron transfer between Clostridium pasteurianum flavodoxin semiquinone and a variety of oxidized homologous cytochromes were measured by using stopped-flow spectrophotometry as previously described (Jung & Tollin, 1981; Simondson et al., 1982; Simondsen & Tollin, 1983). The sources of the various cytochromes used were as specified in an earlier publication (Meyer et al., 1983). Ionic strength was varied by addition of appropriate amounts of NaCl. As was found for horse cytochrome c (Simondsen et al., 1982), the reactions of flavodoxin semiquinone with the other cytochromes used here were biphasic. All of the kinetic data were corrected for this biphasicity as in the previous work, and thus the rate constants reported below refer only to the fast phase of the reaction, which typically represents 70-80% of the total.