Involvement of GDNF and its receptors in the maturation of the periodontal Ruffini endings
Involvement of GDNF and its receptors in the maturation of the periodontal Ruffini endings
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DOI：
10.1016/j.neulet.2006.11.012
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发表时间：
2007-02-02
影响因子：
2.5
通讯作者：
Maeda, Takeyasu
中科院分区：
文献类型：
--
作者：
Igarashi, Yasushi;Aita, Megumi;Maeda, Takeyasu
我们最近的研究发现，幼年大鼠牙周韧带中的主要机械感受器Ruffini末端有强烈的GDNF及其受体的免疫反应。然而，目前还没有关于GDNF及其受体在其发育过程中表达的信息。本研究采用双重免疫荧光染色方法，观察大鼠中牙牙周Ruffini末梢中胶质细胞源性神经营养因子、肾小管上皮细胞生长因子α、L和RET免疫表达的生后变化。出生后3d(PO 3d)，牙周膜内未见GDNF、GFRα、L或RET免疫反应阳性结构。在PO LW，未见GDNF和RET免疫反应的PGP9.5阳性神经纤维呈树突状，周围可见GFRαL反应。PO2w时，粗大轴突和树突状轴突附近可见GDNF阳性终末雪旺细胞，部分轴突呈RET反应，细神经附近无反应细胞。终末雪旺细胞呈GFRαL阳性，但缺乏RET免疫反应。PO3w时，随着牙周Ruffini末梢的形成，GDNF阳性终末雪旺细胞开始增多。这种阶段特异性的免疫表达模式表明，GDNF是牙周Ruffini末端成熟和维持的关键分子。(C)2006爱思唯尔爱尔兰有限公司。保留所有权利。
Our recent study revealed an intense immunoreaction for GDNF and its receptors in the Ruffini endings, primary mechanoreceptors in the periodontal ligament, of young rats. However, no information is available for the expression of GDNF and its receptors during their development. The present study aimed to reveal postnatal changes in the immuno-expression of GDNF, GFR alpha l and RET in the periodontal Ruffini endings of the rat incisors by double immunofluorescent staining. At postnatal day 3 (PO 3d), no structure with GDNF-, GFR alpha l-, or RET-immunoreaction existed in the periodontal ligament. The PGP 9.5-positive nerve fibers without GDNF- and RET-immunoreaction displayed a dendritic fashion at PO lw, with a GFR alpha l-reaction found around these nerves. At PO 2w, GDNF-positive terminal Schwann cells occurred near the thick and dendritic axons, a part of which showed a RET-reaction, with no reactive cells near the thin nerves. The terminal Schwann cells became positive for GFR alpha l, but lacked RET-immunoreaction. At PO 3w, when the formation of the periodontal Ruffini endings had proceeded, GDNF-positive terminal Schwann cells began to increase in number. This stage-specific immuno-expression pattern suggests that GDNF is a key molecule for the maturation and maintenance of the periodontal Ruffini endings. (c) 2006 Elsevier Ireland Ltd. All rights reserved.