Amylase-binding as a discriminator among oral streptococci.
Amylase-binding as a discriminator among oral streptococci.
复制标题
淀粉酶结合作为口腔链球菌的鉴别器。
作者：
C.W.I. Douglas;A. Pease;R. Whiley
对51株血链球菌、米氏链球菌、口腔链球菌及其近缘群的唾液淀粉酶结合能力进行了研究。大多数菌株根据它们与DNA的相关性进行了分组，然后使用14种表型测试进行了比较。S.mitis、S.mior和三个相对较新的菌株群(‘CR’、‘MGH’和‘Tufted mior’)结合唾液淀粉酶，而血链球菌和口腔链球菌不结合唾液淀粉酶。菌株与淀粉酶结合与否的能力在组内非常一致，该测试被证明是可重复性的、快速的和易于执行的。将淀粉酶结合试验与其他6种常规生理试验相结合，可以构建一个二分鉴定检索表，该检索表能够正确识别95%可获得遗传数据的菌株。这些发现表明，微生物结合唾液淀粉酶的能力可能成为某些口腔链球菌鉴定方案中的关键测试。
The ability of 51 strains, belonging to Streptococcus sanguis, 'S. mitior', S. oralis and related groups, to bind salivary amylase was studied. Most strains were grouped according to their DNA-relatedness and then compared using 14 phenotypic tests. S. mitis, 'S. mitior' and three relatively new groups of strains ('CR', 'MGH' and 'Tufted mitior') bound salivary amylase, while strains of S. sanguis and S. oralis did not. The ability of strains to bind amylase or not was remarkably consistent within groups and the test proved to be reproducible, rapid and easy to perform. Combination of the amylase-binding test with 6 other conventional physiological tests allowed the construction of a dichotomous identification key which correctly identified 95% of strains for which genetic data was available. These findings suggest that the ability of organisms to bind salivary amylase could become a key test in identification schemes for certain oral streptococci.