Induced Systemic Resistance by a Plant Growth-Promoting Rhizobacterium Impacts Development and Feeding Behavior of Aphids
Induced Systemic Resistance by a Plant Growth-Promoting Rhizobacterium Impacts Development and Feeding Behavior of Aphids
复制标题
促进植物生长的根际细菌诱导的系统抗性影响蚜虫的发育和摄食行为
作者：
Laurent Serteyn;C. Quaghebeur;M. Ongena;Nuri Cabrera;Andrea Barrera;M. Molina‐Montenegro;F. Francis;C. Ramírez
微生物对植物-昆虫相互作用的影响通常被低估。虽然已知促进植物根际生长的细菌（PGPR）可以诱导植物防御，但食草昆虫寄主的内共生细菌通常对宿主有益。在这里，我们的目的是评估蚕豆植物中 PGPR 诱导的防御是否会影响豌豆蚜，具体取决于豌豆蚜的基因型和内共生体的存在。我们评估了蚜虫的繁殖情况，通过 GC-MS 定量了防御和生长相关的植物激素，并在 PGPR 处理后测量了不同的植物生长和生理参数。此外，我们还通过渗透电图记录了蚜虫的取食行为。我们发现，蚕豆植物的 PGPR 处理减少了其中一种豌豆蚜克隆的繁殖。我们强调了 PGPR 诱导的植物防御启动现象，但没有明显的植物生长促进作用。蚜虫探查行为的主要变化与进入韧皮部筛元件的流涎事件有关。我们认为，内共生体 Hamiltonella defensa 在植物与昆虫相互作用中发挥着关键作用，可能帮助蚜虫抵消植物诱导的抗性，并使它们在 PGPR 处理的植物上正常发育。我们的结果表明，植物和蚜虫相关微生物使蚜虫与植物相互作用的结果更加复杂。
The effects of microorganisms on plant-insect interactions have usually been underestimated. While plant growth-promoting rhizobacteria (PGPR) are known to induce plant defenses, endosymbiotic bacteria hosted by herbivorous insects are often beneficial to the host. Here, we aimed to assess whether PGPR-induced defenses in broad bean plants impact the pea aphid, depending on its genotype and the presence of endosymbionts. We estimated aphid reproduction, quantified defense- and growth-related phytohormones by GC-MS, and measured different plant growth and physiology parameters, after PGPR treatment. In addition, we recorded the feeding behavior of aphids by electropenetrography. We found that the PGPR treatment of broad bean plants reduced the reproduction of one of the pea aphid clones. We highlighted a phenomenon of PGPR-induced plant defense priming, but no noticeable plant growth promotion. The main changes in aphid probing behavior were related to salivation events into phloem sieve elements. We suggest that the endosymbiont Hamiltonella defensa played a key role in plant-insect interactions, possibly helping aphids to counteract plant-induced resistance and allowing them to develop normally on PGPR-treated plants. Our results imply that plant- and aphid-associated microorganisms add greater complexity to the outcomes of aphid-plant interactions.